Lacticaseibacillus rhamnosus Probio-M9 Extends the Lifespan of Caenorhabditis elegans

Probiotics have been characterized as useful for maintaining the balance of host gut flora and conferring health effects, but few studies have focused on their potential for delaying aging in the host. Here we show that Lacticaseibacillus rhamnosus Probio-M9 (Probio-M9), a healthy breast milk probiotic, enhances the locomotor ability and slows the decline in muscle function of the model organism Caenorhabditis elegans. Through attachment to the intestines, Probio-M9 significantly extends the lifespan of C. elegans in a dietary restriction-independent manner. By screening various aging-related mutants of C. elegans, we find that Probio-M9 activates the p38 cascade and daf-2 signaling pathways. Probio-M9 protects and repairs damaged the mitochondria by activating mitochondrial unfolded protein response. We also find that the metabolism of amino acids, sphingolipid, galactose and fatty acids were significantly increased. These changes in metabolites might be involved in extending the lifespan of C. elegans. We reveal Probio-M9 as a dietary supplementation had the potential to delay aging in C. elegans. Similar beneficial effects of Probio-M9 on the human healthspan, such as delaying the occurrence of age-related chronic diseases, might be expected.