Nascent RNA 4sU labelling and enrichment

Abstract 4sU-seq allows for measuring or nascent transcription which can reflect dynamic changes more accurately than traditional RNA-seq. Incorporation of the nucleoside analog 4-thiouridine into RNA allows the separation of nascent transcripts though reversible coupling to biotin and streptavdin bead pull-down. The protocol takes roughly two days to generate a sequencing ready library.