Preparation of Artemisia turcomanic encapsulated niosomal nanocarrier and evaluation of anti-cancer activities and apoptosis gene expression analysis in uterine cancer cell lines (Hela)

AbstractBackgroundArtemisia turcomanicas a natural antibacterial agent, exhibited significant antibacterial effect in the treatment against cancer. Methods This study is the first to investigate size, encapsulation efficiencies, release behavior ofArtemisia turcomanicloaded niosomal nanocarriers, and the anticancer effect of niosomal nanocarriers by MTT assay, flow cytometry, and real time (on Hela cell lines). When the molar ratio of cholesterol: surfactant was 1:2 and the liquid content was 300 µmol, the highest percentage of entrapment efficiency was 83.25%. Moreover, niosomal formulation showed a pH-dependent release; a slow-release profile in physiological pH (7.4), and a more significant release rate at acidic conditions (pH = 5.4). Results In addition, The apoptotic rate ofArtemisialoaded niosomes on Hela cell lines was higher than free extract and pristine niosome. Also, reduction in the expression levels ofBcl2,caspase-3, and p53genes and increase in the expression level ofBAXafter treatment withArtemisia turcomanic-loaded niosomes were more significant than those after treatment with freeArtemisia turcomanicand blank niosome. Conclusion The cytotoxicity results of samples presented thatArtemisia turcomanicloaded niosomes are more beneficial in the death of Hela cell lines.