CRISPR/Cas9 targeted genetic screening in Physcomitrella identifies novel cell division genes

Summary Although plants share core cell division mechanisms with other eukaryotes, their unique features, such as acentrosomal spindle formation and cytokinesis via the phragmoplast, suggest the existence of plant-specific genes. This study used the model bryophyte Physcomitrium patens, commonly known as Physcomitrella, to uncover such genes and employed CRISPR/Cas9-based and localization-based screening to identify novel cell division genes. Co-expression data from known mitotic genes were used to create a pool of 216 candidate genes, which were targeted for CRISPR/Cas9 screening. Frameshift mutants with division defects were characterized using high-resolution imaging and fluorophore-based protein localization. Three novel gene families—CYR (Cytokinesis-Related), LACH (Lagging Chromosome), and SpinMi (Spindle and Phragmoplast Midzone)—were identified. CYR is linked to cytokinesis defects, LACH is essential for chromosome segregation, and SpinMi localizes to the spindle and phragmoplast midzone. None of these gene families has homologs in algae, suggesting their emergence during land colonization. These findings demonstrate the utility of co-expression-guided and targeted screening for discovering genes involved in specific cellular processes and provide insights into the evolution of the plant cell division machinery. Competing Interest Statement The authors have declared no competing interest. Footnotes Added gene models for cyr, spinmi and lach genes, isolated single cyr mutants (Supplemental Figure 2), additional FM4-64 staining (Supplemental Figure 1C, D), and rescue of lach RNAi added (Figure 4)