Ovarian Mesonephric-like Adenocarcinoma: Its Prevalence in a Japanese High-Volume Cancer Center and a Literature Review on Therapeutic Targets.

The present study was conducted in accordance with the Declaration of Helsinki and Good Clinical Practice and with the approval of the Institutional Review Board of the National Cancer Center, Tokyo, Japan (2020-111, 2010-077). Data from January 2010 to June 2023 were used, collecting a total of 683 consecutive patients with ovarian tumors. After the exclusion of 182 cases of borderline tumors, malignant germ cell tumors, or metastatic tumors, 501 cases of primary ovarian cancer were included in this study. At least two board-certified pathologists initially reviewed all the cases, and the pathological diagnoses in this study were subsequently confirmed by a gynecological pathologist (H.Y.) according to the 2020 WHO classification. Pathological diagnoses of the MLA were confirmed based on the previously reported morphological features and immunohistochemical findings [ 4 ]. MLAs should exhibit a morphological similarity to mesonephric adenocarcinoma of the uterine cervix. Different architectural patterns were observed, such as tubular, glandular (pseudo-endometrioid), papillary, cribriform, slit-like, retiform, glomeruloid, and solid, in various combinations. Intraluminal eosinophilic colloid-like material was frequently observed. Metaplastic changes such as squamous, ciliated, and mucinous differentiation are generally absent. Tumor cells are cuboidal or columnar cells with mild or moderately atypical angulated clear vesicular nuclei, often overlapping. The cytoplasm is usually scant to moderate, and mitotic activity is usually conspicuous. Immunohistochemically, the tumor cells show diffuse positivity of PAX8 and focal positivity of TTF-1, GATA3, CD10 (apical/luminal), and calretinin, as well as negativity of ER, PR, and WT-1. A p53 wild-type staining pattern and retained MMR proteins (MSH6 and PMS2) are also observed. One case of ovarian MLA was reported as a single case report [ 6 ]. The criteria for the diagnosis of MLA were summarized in Supplementary Table S1 , and the primary antibodies used for immunohistochemistry for IHC were listed in Supplementary Table S2 . The literature search was performed using publications indexed in PubMed ( http://www.ncbi.nlm.nih.gov/pubmed ) accessed on 29 December 2023 from February 1964 to December 2023. The following search terms were used in the PubMed database: “mesonephric-like adenocarcinoma AND ovary”, or “ovarian mesonephric-like adenocarcinoma”. The reference lists of the included articles were manually checked for any undetected cases. Baseline characteristics were presented as frequencies and proportions for categorical variables. Continuous variables were presented as medians with ranges. All statistical analyses and graphic presentations were performed using SPSS (version 13.0J; SPSS Inc., Chicago, IL, USA).

Mesonephric-like adenocarcinoma (MLA) of the ovary is a newly classified histological subtype added to the 2020 World Health Organization (WHO) classification of female genital tumors. This tumor shares histopathological characteristics with mesonephric adenocarcinoma of the cervix, which originates from mesonephric duct remnants [ 1 ]. Most MLAs are reportedly associated with endometriosis, suggesting that it is one of the tumors related to endometriosis [ 2 , 3 ]. Although MLA has similarities to low-grade endometrioid carcinoma, distinguishing features have been reported in terms of its morphological and immunohistochemical characteristics. Morphologically, the tumor cells, which are cuboidal to columnar, proliferate in various architectural patterns, including tubular, papillary, and solid structures, often containing eosinophilic material within the glandular lumina [ 4 ]. Immunohistochemically, MLA is characterized by positive staining for TTF-1, GATA3, calretinin, and CD10 [ 1 ] and negative staining for estrogen receptor (ER), progesterone receptor (PR), and WT-1. Recognizing this histological type is crucial because, unlike low-grade endometrioid carcinoma, MLAs are reportedly associated with a poor prognosis and show a tendency for advanced stages and early recurrence [ 5 ]. Ovarian MLA is a rare tumor, expected to constitute less than 1% of ovarian tumors, similar to MLA in endometrial carcinoma, but its exact prevalence remains undetermined. Furthermore, reports indicate frequent cases of advanced stages and early recurrences, highlighting the need for therapeutic development. However, knowledge regarding molecular abnormalities that could serve as therapeutic targets in MLA is still insufficient. This study aims to determine the prevalence of ovarian MLA and, by incorporating findings from the existing literature, elucidate its clinicopathological characteristics, focusing on potential therapeutic targets.

Of 501 cases of primary ovarian cancer, MLA was observed in 3 cases (0.6%). Each histological type and its prevalence are presented in Table 1 . The clinical features of these three cases are summarized in Table 2 . The patients’ ages ranged from 52 to 76 years, and all cases were postmenopausal. None of the cases were obese, and the initial symptoms included pelvic pain or the identification of an ovarian tumor due to a pelvic mass. All cases showed elevated CA125 levels, and CA19-9 was elevated in the two cases where it was measured. The maximum tumor diameter on imaging ranged from 13 to 16.5 cm. According to the International Federation of Gynecology and Obstetrics (FIGO, 2008), the initial stages were IC1 in two cases and IIIB in one case. Primary debulking surgery was performed in the two IC1 cases. The IIIB case underwent four courses of dose-dense Paclitaxel Carboplatin (ddTC) therapy as neoadjuvant chemotherapy, followed by interval debulking surgery and two additional courses of ddTC therapy. The initial pathological diagnosis was grade 1 endometrioid carcinoma in one case and mesonephric-like adenocarcinoma (MLA) in two cases. Recurrence was observed postoperatively in both the IC1 cases at 1 and 16 months, respectively. The recurrence sites were the lung in one case and the lung and liver in the other, followed by chemotherapy. The postoperative follow-up period ranged from 8 to 42 months, with outcomes of dead of disease in one case, alive with disease in one case, and no evidence of disease in one case. The pathological features of the three cases are summarized in Table 3 and representative histology is shown in Figure 1 . Figure 1 shows the morphological and immunohistochemical findings of a mesonephric-like adenocarcinoma of the ovary. Macroscopically, all tumors had both solid and cystic components. Histologically, the tumors showed a mixed pattern of glandular and papillary structures, with eosinophilic colloid-like material inside the glands. The tumor cells ranged from cuboidal to columnar, with no nuclear pleomorphism, although some cells exhibited ground glass nuclei and nuclear overlap. Endometriosis was observed in the background of all cases. Immunohistochemically, all cases were positive for GATA3, TTF-1, CD10, and calretinin but negative for the estrogen and progesterone receptors and WT-1. All cases exhibited a wildtype p53 pattern and a retained expression of MMR proteins. Additionally, programmed death-ligand 1 (PD-L1) was negative (combined positive score, CPS < 1). The HER2 score for all tumors was 1+. The commercially available companion HRD test (MyChoice ® ) provided by Myriad Genetics was performed using the ovarian tumor tissue, revealing that homologous recombination repair was proficient in all the cases (genomic instability score was less than five in all the three cases, and neither the BRCA1 nor the BRCA2 pathogenic variant was present). A literature search on PubMed identified 97 cases of ovarian MLA with confirmed pathological diagnoses ( Table 4 and Table 5 ) [ 1 , 5 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ]. The ages ranged from 29 to 84 years. According to FIGO, the initial stages were I in 33 cases, II in 12 cases, III in 15 cases, and IV in 7 cases. A significant proportion (84%, 21/25 cases) were associated with endometriosis. Recurrence was reported in 39% (26/67 cases). A total of 3 patients died among the 23 patients with available follow-up data. Notably, KRAS mutations were observed in 90% (38/42 cases) of the patients. There has been minimal investigation into therapeutic target molecules, and no cases of HRD or MMRd have been identified to date. PD-L1 positivity has also not been reported.

We identified 3 cases (0.6%) of MLA out of the 501 primary ovarian cancer cases based on morphological and immunohistochemical staining results. Two cases showed metastatic recurrence, for which chemotherapy was initiated. All cases were HRP, pMMR, negative for PD-L1 (CPS < 1) and had a HER2 score 1+. The literature review identified 97 previously reported cases, confirming that advanced cases are common and have a high recurrence rate (39%). The molecular features associated with therapeutic targets in these cases were similar to our three cases, but a high frequency of KRAS mutations was observed, suggesting potential targets for therapy. Ovarian MLA is predicted to be a rare tumor among ovarian neoplasms, similar to MLA in endometrial cancer, but there have been no reports on the frequency of ovarian MLA. In contrast, endometrial MLA has been reported to occur at a frequency of approximately 0.7% of all endometrial cancers [ 8 , 33 ]. In a combined morphological and molecular analysis of 570 endometrial carcinomas, only 4 cases (0.7%) were diagnosed as MLA [ 33 ]. Pors et al. (2018) also reported a similar frequency based on an analysis of 585 endometrial carcinomas between 1986 and 2017 [ 8 ]. Our investigation revealed that ovarian MLA, similar to endometrial MLA, occurs at a frequency of 0.6% (3/501, 95% CI 0.12~1.8%) in a Japanese cohort. Additionally, one of our three cases had been diagnosed as grade 1 endometrioid carcinoma. Before the establishment of the MLA concept, some MLA cases might have been diagnosed as low-grade endometrioid carcinoma. In cases of low-grade endometrioid carcinoma that show unusual clinical courses, such as early recurrence or distant metastasis, reconsideration of the diagnosis to MLA might be warranted. Our three cases, along with the 97 cases identified through the literature review, suggest that MLA has more aggressive clinicopathological features compared to low-grade endometrioid carcinoma. Clinically notable, our literature review results show that approximately 12% of low-grade endometrioid carcinomas are reportedly stage III/IV [ 34 ], and 33% of MLAs are at stage III/IV. Additionally, while the recurrence rate of low-grade endometrioid carcinoma is around 9% [ 35 ], 39% of ovarian MLAs are reported to recur based on our literature review results. Furthermore, distant metastasis occurs in 33–56% of MLA cases [ 5 , 15 ]. Among our three cases, one was detected at an advanced stage, and two experienced distant metastatic recurrence. Histopathologically, compared to low-grade endometrioid carcinoma, MLAs typically have fewer solid components and more prominent gland formation. They lack the metaplastic changes such as squamous and mucinous metaplasia which are often seen in endometrioid carcinoma and instead exhibit cellular morphology that is more cuboidal than tall columnar. If immunostaining shows negative hormone receptor status, additional stains such as GATA3, TTF-1, calretinin, and CD10 can aid in diagnosing an MLA [ 4 ]. Consistently, in the molecular classification of endometrial carcinoma (POLEmut, dMMR, p53abn, and NSMP), MLAs display an NSMP profile characterized by wildtype POLE, pMMR, and wildtype TP53, which can further support the diagnosis. Mismatch repair deficiency (dMMR) and PD-L1 expression in tumor cells or the surrounding immune cells are known predictors of the efficacy of immune checkpoint inhibitors across various tumors [ 36 ]. Multiple studies have reported that ovarian MLA exhibits proficient mismatch repair (pMMR) [ 15 , 20 ]. Our literature review also found that all cases with an evaluated MMR status were pMMR (100%, 35/35 cases). In contrast, approximately 8–19% of ovarian endometrioid carcinomas show dMMR [ 37 ], suggesting that pMMR might be a distinguishing feature of MLA from endometrioid carcinoma. However, the lack of increased tumor neoantigen production and immunogenicity in pMMR tumors indicates a lower likelihood of response to immune checkpoint inhibitors. Regarding PD-L1 expression in MLA, only one case has been reported as negative [ 12 ], and similarly, in our cases, PD-L1 expression was minimal in both the tumor and the surrounding immune cells. Although further studies with larger sample sizes are needed, the current evidence suggests that immune checkpoint inhibitors might be ineffective for advanced or recurrent MLA. In our study, we reported an HR status, for the first time, in multiple MLA cases. Previously, only one case had been reported as HRP [ 17 ], and our three cases were also HRP. While approximately half of the high-grade serous carcinoma (HGSC) cases show homologous recombination deficiency (HRD) (Cancer Discovery, 2015), endometriosis-associated ovarian cancers are reported to have lower HRD rates compared to HGSC [ 38 ]. This suggests that ovarian MLA, like other endometriosis-associated ovarian cancers, may predominantly exhibit HRP. Since HRD is a predictive factor for the efficacy of PARP inhibitors, the effectiveness of PARP inhibitors for advanced or recurrent MLA may be limited, given the HRP status. On the other hand, KRAS mutations might be a promising therapeutic target in MLAs. Although we did not test for KRAS mutations in our cases, our literature review found that KRAS mutations were identified in 90% (38/42 cases) of the tested cases, including specific mutations like G12V (42%, 16/38 cases), G12D (39%, 15/38 cases), and G12A (8%, 3/38 cases). Recent developments in targeted therapies for KRAS mutations in the lung and other major cancers suggest potential cross-organ applicability. For example, the KRAS inhibitor sotorasib showed a 37% objective response rate in advanced or recurrent non-small cell lung cancer with KRAS p.G12C mutation [ 39 ]. Although most KRAS mutations in MLA are not p.G12C, ongoing research targeting other KRAS mutations might eventually benefit MLA treatment. Additionally, although our analysis is limited to three cases, all exhibited weak HER2 protein expression (Score 1+). Recent studies have shown the clinical efficacy of HER2-ADC in low-HER2/HER2-expressing cancers, such as breast cancer [ 40 ], indicating that recurrent or metastatic MLA might also be a candidate for such therapies. Beyond HER2, the development of various ADCs is ongoing, and identifying the target molecules in MLA is a crucial future research direction. This study is a retrospective analysis conducted at a single high-volume cancer center, and the disease frequency distribution may differ from that of general hospitals. Therefore, this selection bias may influence the frequency of the MLAs identified in our study. Furthermore, our institution had only three cases to investigate therapeutic target molecules. Hence, the findings need to be validated through multicenter studies with larger cohorts of MLA cases. Additionally, this study did not investigate KRAS gene mutations. Although KRAS mutation testing is not mandatory for diagnosing MLA [ 4 ], identifying KRAS mutations could provide additional support. In summary, an analysis of 501 primary ovarian cancer cases revealed that the frequency of ovarian MLA is 0.6%. Ovarian MLA exhibits HRP, pMMR, PD-L1 negativity, and low-HER2 expression, suggesting that the effectiveness of PARP inhibitors and immune checkpoint inhibitors may be limited. However, the high prevalence of KRAS mutations indicates that KRAS mutation could be a potential therapeutic target for recurrent or metastatic MLA. Given the high incidence of advanced, recurrent, and metastatic cases in MLA, it is essential to validate these therapeutic target findings in larger cohorts.