Performance analysis of different types of cellulase enzyme washed denim using sustainable and conventional washing machine | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Performance analysis of different types of cellulase enzyme washed denim using sustainable and conventional washing machine Md. Mehedi Hasan, Foyej Ahmed, Md. Al Amin This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6933837/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The textile industry continually seeks environmentally friendly and sustainable practices to reduce its ecological footprint. This study investigates the effects of enzymatic wash treatments on denim fabric using both conventional and sustainable machine processes. Enzymes, known for their eco-friendly characteristics, are employed to enhance the aesthetic appeal of denim while minimizing the environmental impact associated with traditional finishing methods. The research involves the application of various enzymes, including Enzymatic DM8639B, Enzymatic DM8642, on denim fabric. Conventional washing machines, commonly used in the textile industry, are compared with innovative sustainable machines designed to optimize water and energy consumption. The study assesses the effectiveness of enzymatic treatments in terms of Washing, Rubbing, and Saliva, Tensile Strength, crucial factors influencing denim quality. Additionally, the environmental impact of both processes is analyzed, considering water usage, energy consumption, and chemical waste generation. Sustainability metrics such as carbon footprint and water footprint are employed to evaluate the eco-friendliness of each method. The findings aim to provide insights into the feasibility of integrating sustainable machine processes into denim production, offering a potential avenue for reducing the environmental impact of garment finishing. This research contributes to the ongoing efforts to develop sustainable practices within the textile industry, aligning with global initiatives for a more environmentally conscious and responsible approach to manufacturing. Chemical Engineering Cellulase enzyme Denim washing Sustainable washing technology Conventional washing machine Textile sustainability Enzymatic treatment Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6933837","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":473792366,"identity":"09216611-9c43-4900-a1de-65c8df951ef9","order_by":0,"name":"Md. 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