Mechanical Stimulation of Red Blood Cells Aging: Focusing on the Microfluidics Application

Human red blood cells (RBCs) are highly differentiated cells essential in almost all physiological processes. During their circulation in the bloodstream, RBCs are exposed to varying levels of shear stress ranging from 0.1-10 Pa under physiological conditions to 50 Pa in arterial stenotic lesions. Moreover, the flow of blood through splenic red pulp and through artificial organs is associated with brief exposure to even higher levels of shear stress, reaching up to hundreds of Pa. As a result of this exposure, some properties of the cytosol, the cytoskeleton, and the cell membrane may be significantly affected. In this review, we aim to systematize the available information on RBC response to shear stress by focusing on reported changes in various red cell properties. We pay special attention to the results obtained using microfluidics since these devices allow the researcher to accurately simulate blood flow conditions in the capillaries and spleen.