MSH6 as a prognostic biomarker in bladder cancer and its correlation with immunity

MSH6 as a prognostic biomarker in bladder cancer and its correlation with immunity | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article MSH6 as a prognostic biomarker in bladder cancer and its correlation with immunity Ning He, Lu-ri Bao, Zhen-li Fu, Wu-niri Gao, Jing-yuan Wang, Li-Gang Chen, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4930584/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 10 Jul, 2025 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract This study aims to investigate the role of MSH6 in the diagnosis and prognosis of bladder cancer and its association with immune responses.Various analyses were conducted on The Cancer Genome Atlas (TCGA) data, and the results were validated using the Gene Expression Omnibus (GEO) database. The potential mechanism of MSH6 in bladder cancer was revealed using R tools, Gene Set Enrichment Analysis (GSEA), and Gene Ontology (GO) analysis. Bladder cancer patients admitted to the Affiliated Hospital of Inner Mongolia Medical University from June 2023 to December 2023 were enrolled. Pathological specimens of bladder cancer and adjacent tissues were obtained. The expression of MSH6 protein in bladder cancer and adjacent tissues was assessed by immunohistochemistry. High expression of MSH6 was identified as an independent prognostic factor for bladder cancer. The occurrence of bladder cancer was also influenced by age, pathological T stage, and pathological stage. According to the Kaplan-Meier survival curve, the overall survival rate of the MSH6 high-expression group was lower (P=0.003). Immunohistochemical results showed the expression of MSH6 in bladder cancer tissues was higher than in adjacent tissues. GSEA revealed a close association between MSH6 and the immune system. MSH6 may play a key role in immune infiltration in bladder cancer. MSH6 is closely related to the diagnosis and prognosis of bladder cancer, suggesting its potential utility as a biomarker for the diagnosis and prognosis of bladder cancer. MSH6 is closely related to the tumor immune microenvironment, implying a significant role in immunotherapy. Biological sciences/Cancer Biological sciences/Immunology Bladder cancer bladder urothelial carcinoma MSH6 Biomarkers Immunization Full Text Additional Declarations No competing interests reported. Supplementary Files Fig.S1.tif Cite Share Download PDF Status: Published Journal Publication published 10 Jul, 2025 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 29 Nov, 2024 Reviews received at journal 27 Nov, 2024 Reviewers agreed at journal 25 Nov, 2024 Reviews received at journal 25 Nov, 2024 Reviewers agreed at journal 25 Nov, 2024 Reviewers invited by journal 24 Nov, 2024 Editor assigned by journal 24 Nov, 2024 Editor invited by journal 02 Sep, 2024 Submission checks completed at journal 30 Aug, 2024 First submitted to journal 17 Aug, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4930584","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":350189969,"identity":"5895fd7a-7730-484c-a9ff-f7204596b5c2","order_by":0,"name":"Ning He","email":"","orcid":"","institution":"the No.2 Hospital of Hohhot","correspondingAuthor":false,"prefix":"","firstName":"Ning","middleName":"","lastName":"He","suffix":""},{"id":350189970,"identity":"df44b414-0892-41f9-a751-c226395376f0","order_by":1,"name":"Lu-ri Bao","email":"","orcid":"","institution":"Inner Mongolia Medical 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urothelial carcinoma, MSH6, Biomarkers, Immunization","lastPublishedDoi":"10.21203/rs.3.rs-4930584/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4930584/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"This study aims to investigate the role of MSH6 in the diagnosis and prognosis of bladder cancer and its association with immune responses.Various analyses were conducted on The Cancer Genome Atlas (TCGA) data, and the results were validated using the Gene Expression Omnibus (GEO) database. The potential mechanism of MSH6 in bladder cancer was revealed using R tools, Gene Set Enrichment Analysis (GSEA), and Gene Ontology (GO) analysis. Bladder cancer patients admitted to the Affiliated Hospital of Inner Mongolia Medical University from June 2023 to December 2023 were enrolled. Pathological specimens of bladder cancer and adjacent tissues were obtained. The expression of MSH6 protein in bladder cancer and adjacent tissues was assessed by immunohistochemistry. High expression of MSH6 was identified as an independent prognostic factor for bladder cancer. The occurrence of bladder cancer was also influenced by age, pathological T stage, and pathological stage. According to the Kaplan-Meier survival curve, the overall survival rate of the MSH6 high-expression group was lower (P=0.003). Immunohistochemical results showed the expression of MSH6 in bladder cancer tissues was higher than in adjacent tissues. GSEA revealed a close association between MSH6 and the immune system. 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