[Culture and identification of microvascular endothelial cells from human endometriosis].

OBJECTIVE: To establish the methods of isolating and culturing human ovarian endometriosis-derived microvascular endothelial cells (OEMEC). METHODS: The tissues of human endometriotic cyst of ovary were finely minced with scissors, then digested by collagenase I, II and trypsin-ethylene diamine tetraacetic acid (EDTA). The cells were purified by using centrifugation of 2000 r/min speed. OEMEC were identified by light microscope and transmission electron microscope observing CD(34), FVIII-Rag and Weibel-Palade in microvascular endothelial cells. RESULTS: The OEMEC grew as confluent monolayer like cobblestones under light microscope. CD(34) and FVIII-Rag were expressed strongly, and the percentages of CD(34) and FVIII-Rag positive cells were 91.4% and 92.5%. Weibel-Palade bodies could be observed under transmission electron microscope. The time of cell doubling proliferation was 4.5 days. CONCLUSION: The established system of isolating OEMEC would provide lab base for studying the mechanisms of angiogenesis in endometriosis lesions.