A Study on Preparation and Characterization of Microcrystalline Cellulose from Lucerne (alfalfa) Waste Fibers

Abstract The preparation and characterization of microcrystalline cellulose (MCC) from processed Lucerne (alfalfa) wastes has been investigated. MCC was prepared by chemical processes including de-waxing, de-lignification, bleaching and acid hydrolysis of Lucerne waste fibers, of which the natural colorants had previously been extracted. Characterization of the prepared MCC was performed and compared with a commercial one (Avicel® PH- 101) in terms of TGA, FTIR, XRD, SEM and Particle Size Analysis. The FTIR spectra of the prepared sample were very similar to those of the commercial sample. The X-ray diffraction pattern showed cellulose crystal lattice with crystallinity index of 78%. The SEM images of prepared MCC had irregular and agglomerated shapes which resemble those of the commercial MCC. The powder was moderately fine, odorless, tasteless and white with particle size distribution of 26 µm, and pH 6.88. The current study showed the feasibility of using Lucerne waste as a low-price and promising source for obtaining MCC.