Effect of Cinnamon and Aconite on Bone Formation-Bone Absorption Coupling in Bone Microenvironment

Aconite and cinnamon can treat many diseases. The study aimed to assess the effectiveness and mechanism of aconite, cinnamon, and aconite-cinnamon in treating osteoporosis. The co-culture system was treated with serum containing aconite, cinnamon, and aconite-cinnamon, and the effects on osteoblast and osteoclast differentiation were evaluated through ALP activity, ALP staining, alizarin red staining, TRAP activity detection, TRAP staining, and F-actin staining. The study also examined the effects of aconite-cinnamon on intercellular factors using ELISA kits, and the effects on different genes were detected through RT-qPCR and Western blotting. Results showed that aconite-cinnamon improved osteoblast differentiation and activity, reduced the release of RANKL, inhibited osteoclast differentiation and activity, and activated the AMPK/SIRT1 signaling pathway in both osteoblasts and osteoclasts. Therefore, aconite-cinnamon can effectively treat and prevent osteoporosis by improving osteogenic differentiation activity and reducing osteoclast differentiation and activity, while activating the AMPK/SIRT1 signaling pathway in both osteoblasts and osteoclasts.