Abstract
Systems and synthetic biology developments often require the construction of many variants of a genetic circuit of interest, resulting in large-scale cloning campaigns. Golden Gate and Modular Cloning (MoClo), two powerful technologies enabling the scale-up of cloning workflows, play a central role for efficient circuit construction. These workflows include a number of dry-lab tasks, which are time-consuming and error-prone at scale. Currently, no software tool is available to handle these tasks in a dedicated, time-saving, and user-friendly manner. We present InSillyClo, an open-source web application to assist large-scale Golden Gate cloning and MoClo workflows. It supports an easy specification of genetic designs at any scale, followed by the automated generation of comprehensive workflow-related data. Moreover, InSillyClo leverages Modular Cloning with a versatile typing system of parts to generate user-defined workflows. InSillyClo is open source, accessible with or without user registration, and can also be used locally.
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Abstract
Systems and synthetic biology developments often require the construction of many variants of a genetic circuit of interest, resulting in large-scale cloning campaigns. Golden Gate and Modular Cloning (MoClo), two powerful technologies enabling the scale-up of cloning workflows, play a central role for efficient circuit construction. These workflows include a number of dry-lab tasks, which are time-consuming and error-prone at scale. Currently, no software tool is available to handle these tasks in a dedicated, time-saving, and user-friendly manner. We present InSillyClo, an open-source web application to assist large-scale Golden Gate cloning and MoClo workflows. It supports an easy specification of genetic designs at any scale, followed by the automated generation of comprehensive workflow-related data. Moreover, InSillyClo leverages Modular Cloning with a versatile typing system of parts to generate user-defined workflows. InSillyClo is open source, accessible with or without user registration, and can also be used locally.
Competing Interest Statement
The authors have declared no competing interest.
Abbreviations
- MoClo
- Modular Cloning
- YTK
- Yeast Tool Kit
- TU
- Transcriptional Unit
- iP
- input Plasmid
- oP
- output Plasmid
- SBOL
- Synthetic Biology Open Language
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