Double Stokes Polarimetric Microscopy for Chiral Fibrillar Aggregates

Double Stokes Polarimetric Microscopy for Chiral Fibrillar Aggregates | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Double Stokes Polarimetric Microscopy for Chiral Fibrillar Aggregates Viktoras Mazeika, Kamdin Mirsanaye, Leonardo Uribe Castano, Serguei Krouglov, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4739645/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 06 Feb, 2025 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract Second harmonic generation (SHG) microscopy is a powerful tool for imaging collagen and other noncentrosymmetric fibrillar structures in biological tissue. Polarimetric SHG measurements provide ultrastructural information about the fibrillar organization in a focal volume (voxel). We present a reduced nonlinear polarimetry method named double Stokes polarimetry (DSP) for quick characterization of chiral C6 symmetry fibers without data fitting that simplifies and speeds up the polarimetric analysis. The method is based on double Stokes-Mueller polarimetry and uses linear and circular incident and outgoing polarization states. The analytical expressions of DSP polarimetric parameters are defined in terms of conventional SHG Stokes vector components. A complex chiral susceptibility (CCS) model is assumed to derive expressions of ultrastructural parameters consisting of the magnitude and phase of molecular complex-valued chiral susceptibility ratio, real-valued achiral ratio, and fiber orientation in a voxel. The ultrastructural parameters are expressed in terms of directly measurable DSP polarimetric parameters. DSP is validated with rat tail tendons sectioned at different orientations. DSP can be applied to investigate the origin of chiral complex-valued susceptibility of collagen, to study modifications of collagen in cancerous tissue, to map ultrastructural parameters of large areas for whole-slide histopathology, and to image moving specimens in real time. Physical sciences/Physics/Techniques and instrumentation/Microscopy/Multiphoton microscopy Physical sciences/Optics and photonics/Optical techniques/Microscopy/Polarization microscopy Physical sciences/Physics/Biological physics Physical sciences/Physics/Optical physics/Nonlinear optics Full Text Additional Declarations No competing interests reported. Supplementary Files MazeikaSupplementaryMaterial.pdf Cite Share Download PDF Status: Published Journal Publication published 06 Feb, 2025 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 17 Sep, 2024 Reviews received at journal 11 Sep, 2024 Reviewers agreed at journal 01 Sep, 2024 Reviews received at journal 28 Aug, 2024 Reviewers agreed at journal 15 Aug, 2024 Reviewers invited by journal 15 Aug, 2024 Editor assigned by journal 05 Aug, 2024 Editor invited by journal 29 Jul, 2024 Submission checks completed at journal 24 Jul, 2024 First submitted to journal 14 Jul, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4739645","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":342527939,"identity":"5b1e001f-152d-4593-b381-4bebc59c5675","order_by":0,"name":"Viktoras Mazeika","email":"","orcid":"","institution":"Vilnius University","correspondingAuthor":false,"prefix":"","firstName":"Viktoras","middleName":"","lastName":"Mazeika","suffix":""},{"id":342527940,"identity":"8c0c0b1c-ec44-4d35-99f3-90d14c6757e0","order_by":1,"name":"Kamdin Mirsanaye","email":"","orcid":"","institution":"University of 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Polarimetric SHG measurements provide ultrastructural information about the fibrillar organization in a focal volume (voxel). We present a reduced nonlinear polarimetry method named double Stokes polarimetry (DSP) for quick characterization of chiral C6 symmetry fibers without data fitting that simplifies and speeds up the polarimetric analysis. The method is based on double Stokes-Mueller polarimetry and uses linear and circular incident and outgoing polarization states. The analytical expressions of DSP polarimetric parameters are defined in terms of conventional SHG Stokes vector components. A complex chiral susceptibility (CCS) model is assumed to derive expressions of ultrastructural parameters consisting of the magnitude and phase of molecular complex-valued chiral susceptibility ratio, real-valued achiral ratio, and fiber orientation in a voxel. The ultrastructural parameters are expressed in terms of directly measurable DSP polarimetric parameters. 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