The effects of cryopreservation on follicular fluid samples in patients with Polycystic Ovary Syndrome

Purpose: To investigate the quality control of follicular fluid samples from polycystic ovary syndromepatients under -80 ℃ cryopreservation, explore the effects of cryopreservation in differentperiods. Methods: Seventy-five samples were selected to evaluate the quality of PCOS samples stored in a -80°C refrigerator at the Northern Jiangsu People’s Hospital during different time periods (from 2017 to 2018) by DNA integrity determination, RNA integrity determination and housekeeping gene expression. Results: The samples of PCOS follicles were divided into groups to be tested at five stages, with each stage lasting four months. The DNA quality of all the samples was high and without significant degradation in the majority of samples (97%). Samples had high RNA quality, and they fully satisfied the scientific research requirements with RINs≥6. Conclusion: The majority of samples had high molecular quality and were suitable for the requirements of downstream experiments. This study provides a reference for DNA and RNA extraction from different PCOS samples preserved for different amounts of time for scientific research.