Flow cytometry study on the effect of serum and peritoneal fluid of women on sperm-binding activity of immunoglobulin G antisperm antibodies

Marina A. Nikolaeva, M. A. Nikolaeva, Kulakov Vi, V. I. Kulakov, Goukasian IA, I A Goukasian, Irina A. Goukasian, R D Philippova, Roza D. Philippova, Philippova RD, Korotkova IV, I. V. Korotkova, Sukhikh GT, G T Sukhikh, Г. Т. Сухих
Fertility and sterility · 1997 · vol. 67(4) , pp. 680–686 · doi:10.1016/s0015-0282(97)81366-0 · PMID:9093194 · W2053838341
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This flow cytometry study investigated the impact of serum and peritoneal fluid from women on the sperm-binding activity of immunoglobulin G antisperm antibodies.

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Abstract

ObjectiveTo investigate the influence of sera and peritoneal fluids (PFs) from fertile and infertile women on the binding of antisperm antibodies to the surface of spermatozoa.DesignThe immunoglobulin (Ig) G antisperm antibodies binding to the surface of liver spermatozoa was evaluated after their incubation in antisperm antibodies-positive serum from an infertile male in the presence and absence of female sera or PFs.SettingRussian Scientific Center for Obstetrics, Gynaecology, and Perinatology.Patient(s)Serum and PF samples from fertile and infertile women; antisperm antibodies-positive serum from infertile men; high-quality fresh semen from healthy donors.Intervention(s)Serum samples were obtained from fertile and infertile women and from infertile men. Peritoneal fluids were collected during routine laparoscopy.Main outcome measure(s)The proportion of spermatozoa positive for IgG antibodies and the quantity of antisperm antibodies on the sperm surface measured by flow cytometry (FCM).Result(s)The addition of sera from fertile or infertile women with endometriosis or pelvic adhesion disease to an IgG antisperm antibodies-positive male serum resulted in significant inhibition of the antisperm antibodies binding to the sperm surface.Conclusion(s)Sera of fertile as well as infertile woman contain factors that block IgG antisperm antibodies binding to the surface of live spermatozoa.

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MeSH descriptors

Blood Proteins Flow Cytometry Immunoglobulin G Infertility, Female Spermatozoa Spermatozoa Ascitic Fluid Ascitic Fluid Ascitic Fluid Ascitic Fluid Binding, Competitive Binding, Competitive Blood Proteins Blood Proteins Blood Proteins Female Fluorescein-5-isothiocyanate Fluorescein-5-isothiocyanate Fluorescence Fluorescent Dyes

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