Tunable gene expression in zebrafish using RiboSCALE

ABSTRACT Chemogenetic tools enable conditional control of gene expression during embryonic development and regeneration. However, many conditional tools induce constitutive or one-way activity precluding temporal resolution of gene function or require the use of multiple transgenic lines. We developed an RNA-based chemogenetic approach to induce gene expression in zebrafish embryos and larvae. We demonstrate that a gene of interest can be turned on in a time-dependent and concentration-dependent manner. Using this approach, we have characterized two different aptamers for future investigation. Competing Interest Statement The authors have declared no competing interest. Funder Information Declared National Institutes of Health, https://ror.org/01cwqze88, R21GM143565, R03NS136719 Natural Sciences and Engineering Research Council, RGPIN-2026-06146 Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.