Epithelial-mesenchymal transition and anoikis resistance induced by Ang2 in salivary adenoid cystic carcinoma

Epithelial-mesenchymal transition and anoikis resistance induced by Ang2 in salivary adenoid cystic carcinoma | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Epithelial-mesenchymal transition and anoikis resistance induced by Ang2 in salivary adenoid cystic carcinoma Zheng Min, Haihui Zhao, Xiaojuan Zhong, Guo Jun, Zhang Ping, Wei Fei, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4780785/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Patients, who suffer from Salivary Adenoid Cystic Carcinoma (SACC), face a heightened risk of recurrent or metastatic disease attributed to significant neuroinvasion, even with standard treatment, highlighting the imperative for innovative treatment targets and approaches. Our research indicates that the expression of angiopoietin-2 (Ang2) in the cytoplasm and nucleus of SACC tissue is significantly higher than that of normal tissue adjacent to cancer, which is related to the pathological type of the tissue. Approximately 74.2% of SACC express Ang2, which promotes survival and lung metastasis. In SACC cells, downregulation of Ang2 inhibits cell migration and enhances cell apoptosis, and resistance to cell apoptosis is deemed essential for metastasis. The downregulation of Ang2 leads to the restoration of E-cadherin levels by facilitating the proteasome-dependent degradation of its suppressor Snail.Overexpression of Snail prevented the complete inhibitory effect of Ang2 on ACC cell migration, which was in line with the promotion of epithelial mesenchymal transition (EMT). The in vivo findings demonstrated reduced lung colonization in conjunction with the downregulation of Ang2. Our findings illustrate Ang2 triggers EMT and enhances metastasis in SACC, suggesting that Ang2 could act to be a promising therapeutic option towards individuals with SACC. Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4780785","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":335286596,"identity":"8ec06f58-4715-4e25-b8ec-0845fbb9bc91","order_by":0,"name":"Zheng Min","email":"","orcid":"","institution":"ZhouShan Hospital, Wenzhou Medical University","correspondingAuthor":false,"prefix":"","firstName":"Zheng","middleName":"","lastName":"Min","suffix":""},{"id":335286599,"identity":"69168132-62c3-4ad3-b635-8d2f5d8abfdb","order_by":1,"name":"Haihui Zhao","email":"","orcid":"","institution":"Southwest Medical 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