Investigation of patient-derived endometriosis in a 3D-in-vivo-model

Philipp E. Scherer, Nina Kurz-Mammri, Erin H. Graf, Thomas Papathemelis, R Donutiu, Sabrina Harteis, Christopher Kohl
In: Geburtshilfe und Frauenheilkunde · 2024 · vol. 84(10) , pp. e220 · doi:10.1055/s-0044-1790966 · W4403327702
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This study investigated patient-derived endometriosis using a 3D-in-vivo model to explore its behavior and characteristics in a simulated environment.

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This study utilized the chorioallantoic membrane model to evaluate the behavior of patient-derived endometriosis tissue in a 3D-in-vivo setting. Researchers measured vascularization changes via laser speckle contrast analysis and assessed cellular proliferation through immunohistochemical staining for Ki67. Preliminary results demonstrated increased vascularization and sustained proliferative activity, indicating that the engrafted tissue remained vital within the model. The authors note that comparative analyses with clinical data and the specific effects of chicken estrogen are currently under investigation. This paper is centrally about endometriosis — specifically the development and validation of an experimental model using patient-derived lesions.

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Material

The chorioallantoic membrane (CAM) model was used to establish a 3D-in-vivo-model for the investigation of endometriosis. Endometriosis tissue was obtained from patients undergoing surgery at an EndoCert certified hospital.

Methods

Changes in the vascularization of the engrafted tissue on the CAM were measured over time using laser speckle contrast analysis (LASCA). The fertilized chicken eggs used for the CAM model were stratified for their gender utilizing an in ovo sexing technique. To test for changes of molecular markers throughout the engraftment period, immunohistochemical staining was performed. Patient-related data was analyzed in retrospect using the AGEM questionnaire.

Results

Preliminary data show an increase in vascularization of the engrafted tissue on the CAM. The analysis of Ki67 shows proliferative activity of endometriosis cells at the end of the experiment. The comparative results between laboratory and clinical data and the effect of chicken estrogen on the investigated endometriosis tissue are under current investigation. Summary: Angiogenesis as one of the major pathologic features of endometriosis can be measured in the 3D-in-vivo-model via an increase in vascularization. As the patient-derived tissue remained vital the model can potentially be used for the testing of drugs and their effect on proliferation, angiogenesis, and molecular markers, amongst others. Publication History Article published online: 01 October 2024 © 2024. Thieme. All rights reserved. Georg Thieme Verlag KG Rüdigerstraße 14, 70469 Stuttgart, Germany

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endometriosis

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