The dimethyloxalylglycine-functionalized nanofibers for in situ regeneration of infected developing dental roots

The dimethyloxalylglycine-functionalized nanofibers for in situ regeneration of infected developing dental roots | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article The dimethyloxalylglycine-functionalized nanofibers for in situ regeneration of infected developing dental roots Kyung Mi Woo, Yeon-Jee Yoo, Lee Eun-Hye, Jieun Bae, Woo Jin Kim, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4180004/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract In situ regeneration in restorative dentistry focuses on repairing tissues directly at the injury site by using engineered biomaterials to guide endogenous cell activity. This approach aims to simplify treatment processes and improve outcomes for conditions like developing teeth with necrotic pulp infections. This study explores the potential of poly(ε-caprolactone) fibers (PCLF) functionalized with dimethyloxalylglycine (DMOG) for in situ regeneration in the context of dental root repair in immature teeth with necrotic pulp and apical periodontitis. In vivo application to a model in beagle dogs demonstrated the effectiveness of PCLF/DMOG in promoting root development, apical closure, and clearing infectious lesions, contrasting with calcium hydroxide paste, contemporary regenerative endodontic treatment controls that showed thin root growth and resilient persistent infection. Mechanistically, the sustained release of DMOG from PCLF/DMOG significantly enhanced the expression of hypoxia-inducible factor 1-alpha and upregulated genes associated with angiogenesis and neurogenesis, including vascular endothelial growth factor-A and nerve growth factor. The PCLF/DMOG upregulated antimicrobial peptides, facilitated efferocytic activities, and promoted macrophage polarization to the M2 phenotype. These findings highlight the potential of PCLF/DMOG scaffolds for dental root regeneration, offering a promising approach for treating immature teeth with necrotic pulp and apical periodontitis through in situ regeneration. Biological sciences/Biotechnology/Tissue engineering Biological sciences/Biotechnology/Biomaterials/Bioinspired materials in situ regeneration infected developing dental roots dimethyloxalylglycine nanofibers HIF-1α macrophages antimicrobial peptides Full Text Additional Declarations (Not answered) Supplementary Files SupplementaryMaterial.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4180004","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":286993964,"identity":"2837a902-c160-4b79-8bfb-796b49bb611c","order_by":0,"name":"Kyung Mi 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