Genome of a Novel Freshwater Microcystis Cyanophage Mwe-Yong1112-1

Abstract Freshwater Microcystis cyanophage Mwe-Yong1112-1 is the first virus isolated with M. wesenbergii. The complete genome of the cyanophage is 39,679 bp in length with a G+C content of 66.6%. BLASTx scanning results indicated “No significant similarity found”. Mwe-Yong1112-1 shared the highest pair-wise average nucleotide identity (ANI) value of 60.83% (below the ≥95% boundary to define a species) and the highest nucleotide sequence similarity of 17.48% (below the >50% boundary to define a genus) with the closest phage in pairwise sequence comparison (PASC). In the proteomic tree, Mwe-Yong1112-1 formed an independent branch in Siphoviridae family. Analysis indicated that cyanophage Mwe-Yong1112-1 should be considered as a novel phage presenting a novel genus in Siphoviridae family.