Plasma Trimethylamine N-Oxide as a Non-Invasive Biomarker for Adenomyosis: A Cross-Sectional Study

Plasma Trimethylamine N-Oxide as a Non-Invasive Biomarker for Adenomyosis: A Cross-Sectional Study | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 5 August 2025 V1 Latest version Share on Plasma Trimethylamine N-Oxide as a Non-Invasive Biomarker for Adenomyosis: A Cross-Sectional Study Authors : Yuhang Chen , Xiaoyu Li , Xun Yuan 0009-0006-0185-1650 , Anqi Qin , Yu Liao , Xinming Wang , Shuang Ling , Xiangbing Zeng , and Jinlong Qin [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.175439363.37991172/v1 167 views 97 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Objective To identify novel and noninvasive biomarkers for the diagnosis of adenomyosis and to provide potential therapeutic targets. Design We conducted a cross-sectional study using data from 110 women of reproductive age who underwent pelvic examinations, including patients with adenomyosis, endometriosis, and healthy controls. Setting Hospital-based, at Shanghai Fourth People’s Hospital between October 2021 and October 2022. Sample Women of reproductive age who underwent pelvic examinations. Methods 110 women of reproductive age were initially included in this study. Blood routine test results and plasma metabolite levels were compared among healthy controls, patients with endometriosis, and patients with adenomyosis. Plasma phenylacetylglutamine (PAGln) and trimethylamine N-oxide (TMAO) levels were detected using a Thermo Scientific TSQ Quantis triple quadrupole mass spectrometer. Main Outcome Measures Plasma levels of trimethylamine N-oxide (TMAO) and phenylacetylglutamine (PAGln) were the main laboratory outcome measures, while age, gravidity, and complete blood count parameters served as the main clinical outcome measures. Results Among the 110 women, 25 healthy controls, 21 with endometriosis, and 28 with adenomyosis were included in this study. We found that plasma TMAO levels, but not PAGln levels, were specifically higher in the adenomyosis group (adenomyosis vs. Healthy Control: p = 0.029; adenomyosis vs. endometriosis: p = 0.038; endometriosis vs. Healthy Control: p = 0.999). After filtering women aged 30–40 years, the TMAO level in the adenomyosis group remained higher than that in the endometriosis group (p = 0.016). Conclusions Increased plasma TMAO levels may be a potential predictor for the diagnosis and treatment of adenomyosis. Supplementary Material File (figures.docx) Download 479.14 KB File (manuscript.docx) Download 100.45 KB File (tables.docx) Download 36.62 KB Information & Authors Information Version history V1 Version 1 05 August 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords adenomyosis: diagnosis basic science Authors Affiliations Yuhang Chen Shanghai Fourth People's Hospital View all articles by this author Xiaoyu Li Shanghai University of Traditional Chinese Medicine View all articles by this author Xun Yuan 0009-0006-0185-1650 Tongji University School of Life Sciences and Technology View all articles by this author Anqi Qin Tongji University School of Life Sciences and Technology View all articles by this author Yu Liao Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital View all articles by this author Xinming Wang Shandong Academy of Chinese Medicine View all articles by this author Shuang Ling Shanghai University of Traditional Chinese Medicine View all articles by this author Xiangbing Zeng Shanghai University of Traditional Chinese Medicine View all articles by this author Jinlong Qin [email protected] Shanghai Fourth People's Hospital View all articles by this author Metrics & Citations Metrics Article Usage 167 views 97 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Yuhang Chen, Xiaoyu Li, Xun Yuan, et al. Plasma Trimethylamine N-Oxide as a Non-Invasive Biomarker for Adenomyosis: A Cross-Sectional Study. Authorea . 05 August 2025. DOI: https://doi.org/10.22541/au.175439363.37991172/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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