Overexpression of SHCBP1 predicts poor prognosis of gastric cancer

Background: SHC SH2-domain binding protein 1 (SHCBP1) functions as an oncogene in different cancers. The effect pattern of SHCBP1 in gastric cancer (GC) tissues and the relationships between SHCBP1 expression and GC progression were explored in our study. Methods The expression of SHCBP1 in GC tissues and corresponding adjacent tissues was analyzed by immune histochemistry, Western blot and RT-PCR. With bioinformatics methods, the associations between the expression level of SHCBP1 and different cancer progression were also analyzed. In addition, the knockdown of SHCBP1 gene using shRNA transfection was applied to investigate the effect of SHCBP1 expressionon the proliferation, migration, invasion and epithelial to mesenchymal transition (EMT) capabilities of GC cells. Results The expression level of SHCBP1 mRNA was significantly increased in GC tissues compared with that of the adjacent tissues (P < 0.05). The expressions of SHCBP1 in GC tissues were associated with T stage and TNM stage. High SHCBP1 expression and TNM stage were independent factors to predict poor prognosis for GC patients.With the bioinformatics database, SHCBP1 could be treated as an oncogene of GC. And the in vitro study further confirmed that the knockdown of SHCBP1 expression in GC cells effectively suppressed the proliferation, migration, invasion and EMT capabilities of GC cells. Conclusions Our results indicate that SHCBP1 promote the metastasis of GC and could be served as potential prognostic marker and potential molecular target for GC therapy.