Bioethanol production from paper sludge containing antiseptic and deinking agent by subcritical water pretreatment and semi-simultaneous saccharification and fermentation

Bioethanol production from paper sludge containing antiseptic and deinking agent by subcritical water pretreatment and semi-simultaneous saccharification and fermentation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Bioethanol production from paper sludge containing antiseptic and deinking agent by subcritical water pretreatment and semi-simultaneous saccharification and fermentation Idzumi Okajima, Masato Muto, Shingo Morimoto, Kazuki Nauchi, Yuta Kodama, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3909044/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 4 You are reading this latest preprint version Abstract For a long time, bioethanol production from wood-based biomass has been investigated. However, most studies face issues including high costs and technical difficulties associated with delignification and saccharification. Paper sludge (PS) from paper mills has a significant potential for bioethanol production. In this study, waste-paper-containing PS is used as the raw material for bioethanol production because the annual waste paper utilization rate has increased. Although PS does not require delignification, the antiseptics and deinking agents in PS inhibit enzymatic reactions such as saccharification and fermentation and their removal has not yet been reported. Using subcritical water pre-treatment, the selective decomposition of enzyme inhibitors in PS is examined without the generation of other enzyme inhibitors. The optimum pretreatment conditions are identified as 240°C, 3.3 MPa, 3 min, and pH 4.5. Glucose was obtained in 71% yield from pretreated 5 wt% PS using cellulase, which is 5.5 times higher than that from unpretreated PS. This is because the reactivity of the pretreated PS increases with the surface area of the cellulose fibers, and the cellulase inhibitors are decomposed by subcritical water. Next, semi-simultaneous saccharification and fermentation treatments are performed to produce bioethanol from waste-paper-containing PS. The bioethanol yield based on cellulose after 96 h is 68% for PS pretreated with subcritical water, whereas the bioethanol yield is 6% for unpretreated PS. Therefore, subcritical water pretreatment increases the bioethanol yield by 11 times. Proposed method allows large amount of PS to be used as ethanol feedstock in future. bioethanol glucose paper sludge subcritical water pretreatment antiseptic Full Text Cite Share Download PDF Status: Under Review Version 1 posted Reviewers agreed at journal 29 Jan, 2024 Reviewers invited by journal 29 Jan, 2024 Editor assigned by journal 29 Jan, 2024 First submitted to journal 28 Jan, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3909044","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":270181040,"identity":"d4d11858-e65c-48a6-9be2-06fe12123fa4","order_by":0,"name":"Idzumi Okajima","email":"","orcid":"","institution":"Shizuoka Daigaku","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Idzumi","middleName":"","lastName":"Okajima","suffix":""},{"id":270181041,"identity":"6309d669-7c32-4e67-8797-5b004e7a6306","order_by":1,"name":"Masato Muto","email":"","orcid":"","institution":"Shizuoka Daigaku","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Masato","middleName":"","lastName":"Muto","suffix":""},{"id":270181042,"identity":"8418f35d-953d-4a65-a6d5-743518a48643","order_by":2,"name":"Shingo Morimoto","email":"","orcid":"","institution":"Shizuoka Daigaku","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Shingo","middleName":"","lastName":"Morimoto","suffix":""},{"id":270181043,"identity":"b4c496b8-67e5-496c-9d1a-748bfab6b5fe","order_by":3,"name":"Kazuki Nauchi","email":"","orcid":"","institution":"Shizuoka Daigaku","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Kazuki","middleName":"","lastName":"Nauchi","suffix":""},{"id":270181044,"identity":"ce42d198-0b62-4332-8a30-4622300c340f","order_by":4,"name":"Yuta Kodama","email":"","orcid":"","institution":"Shizuoka Daigaku","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yuta","middleName":"","lastName":"Kodama","suffix":""},{"id":270181045,"identity":"5632e430-6a90-4047-89fc-a28697a7ab50","order_by":5,"name":"Enoch Y. 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However, most studies face issues including high costs and technical difficulties associated with delignification and saccharification. Paper sludge (PS) from paper mills has a significant potential for bioethanol production. In this study, waste-paper-containing PS is used as the raw material for bioethanol production because the annual waste paper utilization rate has increased. Although PS does not require delignification, the antiseptics and deinking agents in PS inhibit enzymatic reactions such as saccharification and fermentation and their removal has not yet been reported. Using subcritical water pre-treatment, the selective decomposition of enzyme inhibitors in PS is examined without the generation of other enzyme inhibitors. The optimum pretreatment conditions are identified as 240\u0026deg;C, 3.3 MPa, 3 min, and pH 4.5. Glucose was obtained in 71% yield from pretreated 5 wt% PS using cellulase, which is 5.5 times higher than that from unpretreated PS. This is because the reactivity of the pretreated PS increases with the surface area of the cellulose fibers, and the cellulase inhibitors are decomposed by subcritical water. Next, semi-simultaneous saccharification and fermentation treatments are performed to produce bioethanol from waste-paper-containing PS. The bioethanol yield based on cellulose after 96 h is 68% for PS pretreated with subcritical water, whereas the bioethanol yield is 6% for unpretreated PS. Therefore, subcritical water pretreatment increases the bioethanol yield by 11 times. 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