Evaluation of Mixture Magnesium Oxide and Zinc Oxide Nanoparticles against Multi-drug-resistance Mycobacterium tuberculosis by Microplate Alamar Blue Assay

Abstract Objective The current study is the first experimental study of which has evaluated the MICs and MBCs of ZnONPs, MgONPs, and mixture MgONPs-ZnONPs against H 37 Rv Mtb and MDR-Mtb.Results The MIC of MgONPs and ZnONPs were 0.195 and 0.468 µg.ml -1 against 10 4 of H 37 Rv Mtb. As well, 0.166 µg.ml -1 of MgONPs-ZnONPs was able to inhibit 10 -4 H 37 Rv Mtb. The MIC of MgONPs against 10 4 concentrations of MDR-Mtb was 12.5 µg.ml -1 . The MIC of MgONPs/ZnONPs against 10 4 concentrations of MDR-Mtb reached to 0.664 µg.ml -1 . Based on the results, the MBC value of ZnONPs increased to 1.875 µg.ml -1 against 10 -4 concentrations of MDR-Mtb. Testing showed that the MBCs of MgONPs/ZnONPs reached to 1.328 µg.ml -1 against 10 4 concentrations of MDR-Mtb. The half maximal inhibitory concentration (IC50) against MDR-TB was 0.779 µg.ml -1 for ZnONPs and 0.883 µg.ml -1 for MgONPs-ZnONPs. The MgONPs-ZnONPs was not toxic to Vero cell lines however ZnONPs could inhibit the Vero and HepG 2 cell lines. We found that ZnONPs and mixture MgONPs-ZnONPs not only have higher bactericide behavior but might have also synergistic effects against MDR-TB.