Shoot organogenesis and plant regeneration from stem explants in Acer truncatum

Shoot organogenesis and plant regeneration from stem explants in Acer truncatum | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Shoot organogenesis and plant regeneration from stem explants in Acer truncatum Yiming Du, Wenqi Zhao, Meng Xin, Shushun Li, Lu Zhu, Qiuyue Ma, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7343932/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 18 Dec, 2025 Read the published version in Plant Cell, Tissue and Organ Culture (PCTOC) → Version 1 posted 4 You are reading this latest preprint version Abstract Acer truncatum is an economically important multipurpose tree in China due to its high values in ornamental, ecology, oil-production and medicine. The lack of reliable and stable in vitro regeneration system seriously restricts its breeding and industrial development. This study first presented a protocol for in vitro regeneration of A. truncatum via de novo shoot organogenesis from stem explants. The results showed that sterilization, basal medium, plant growth regulators and light condition significantly influenced shoot regeneration of A. truncatum . The best sterilization protocol for stem explants was 75% ethanol (30 s) + 0.1% HgCl 2 (10 min ), with a lowest contamination rate (4.74%). The highest callus induction rate (97.17%) was achieved when stem segments were cultured on WPM medium with 0.2 mg/L 2,4-D and 0.5 mg/L 6-BA in the dark for 15 days. The optimal shoot differentiation rate (10.51%) was obtained when callus was cultured on NN69 medium containing 0.5 mg/L CPPU. The rooting rate of adventitious shoots was achieved 85% by using NN69 medium with 0.25 mg/L IBA, and the rooted seedlings were transplanted into pots containing a mixture of peat, perlite and vermiculite (2:1:1, v:v:v), the survival rate was more than 80%. Histological analysis demonstrated the origin and development process of indirect shoot organogenesis, including callus formation, meristematic nodule formation and shoot differentiation. This study will be beneficial to the germplasm conservation, mass propagation and genetic improvement of A. truncatum . Acer truncatum Shoot organogenesis In vitro regeneration Histology Full Text Supplementary Files Graphicalabstract.tiff Highlights.docx Cite Share Download PDF Status: Published Journal Publication published 18 Dec, 2025 Read the published version in Plant Cell, Tissue and Organ Culture (PCTOC) → Version 1 posted Reviewers agreed at journal 23 Aug, 2025 Reviewers invited by journal 21 Aug, 2025 Editor assigned by journal 14 Aug, 2025 First submitted to journal 12 Aug, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7343932","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":503560054,"identity":"5656b350-7ca1-48d8-af9b-f6ffcbc5d7c1","order_by":0,"name":"Yiming Du","email":"","orcid":"","institution":"Jiangsu Academy of Agricultural Sciences","correspondingAuthor":false,"prefix":"","firstName":"Yiming","middleName":"","lastName":"Du","suffix":""},{"id":503560055,"identity":"71b5cde7-b1a7-4c47-9137-fb1fdf98283c","order_by":1,"name":"Wenqi Zhao","email":"","orcid":"","institution":"Hebei Normal University of Science and 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