Integrative Taxonomic Delimitation of the Endemic Thlaspi syriacum and Thlaspi microstylum Using Molecular, Micromorphological, and Anatomical Evidences

Integrative Taxonomic Delimitation of the Endemic Thlaspi syriacum and Thlaspi microstylum Using Molecular, Micromorphological, and Anatomical Evidences | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Ecology and Evolution This is a preprint and has not been peer reviewed. Data may be preliminary. 12 May 2026 V1 Latest version Share on Integrative Taxonomic Delimitation of the Endemic Thlaspi syriacum and Thlaspi microstylum Using Molecular, Micromorphological, and Anatomical Evidences Authors : Mehmet Karaismailoğlu 0009-0007-7363-1478 [email protected] [email protected] , Vahap Eldem [email protected] , Shaima Abdulrahman [email protected] , Behcet inal [email protected] , Mehmet Balcı [email protected] , and Mehmet Cengiz KARAİSMAİLOĞLU [email protected] Authors Info & Affiliations https://doi.org/10.22541/authorea.15003116/v1 9 views 4 downloads Contents Abstract Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Thlaspi L. sensu lato (Brassicaceae)is a taxonomically problematic genus characterized by high morphological similarity and unclear species boundaries. This is particularly evident in the center of diversity in Anatolia. The species Thlaspi microstylum Boiss. and T. syriacum Bornm. have long been subject to inconsistent taxonomic interpretations due to their overlapping macromorphological characters. In this study, the two closely related species were reevaluated using a comprehensive approach combining full chloroplast genome sequencing, genome skimming-based phylogenomic analyses, chloroplast microsatellite studies, flower micromorphology, and petiole anatomy. The plastomes of both species were sequenced for the first time, and comparative analyses confirmed the highly conserved quadripartite genome structure typical of Brassicaceae. However, taxonomically informative fine-scale differences were detected in IR boundary configurations and the structural state of the ycf15 gene. cpSSR analyses revealed species-specific microsatellite profiles and, particularly in T. syriacum, a higher AT-repeat diversity provided potential markers for future population genetics and phylogeography studies. Alignment-free k-mer-based phylogenetic analyses and plastid average nucleotide identity (ANI) results supported both taxa as mutually monophyletic and genomically distinct lineages, despite intraspecific variation. Scanning electron microscopy revealed distinctive micromorphological differences in the surface ornamentation of petals, ovaries, and styles, while stable characters such as petiole anatomy, the number of vascular bundles, petiole thickness, and surface configuration reinforced species differentiation. The strong concordance between genomic, micromorphological, and anatomical data indicates that T. microstylum and T. syriacum represent independent evolutionary units. This study demonstrates the effectiveness of comprehensive systematic approaches for resolving taxonomic uncertainties in morphologically problematic plant groups and contributes to a more robust, evolutionarily coherent classification of Thlaspi sensu lato. Information & Authors Information Version history V1 Version 1 12 May 2026 Collection Ecology and Evolution Authors Affiliations Mehmet Karaismailoğlu 0009-0007-7363-1478 [email protected] [email protected] Bartin Universitesi, Bartın, Turkey, 74100 View all articles by this author Vahap Eldem [email protected] Bartin Universitesi, Bartın, Turkey, 74100 View all articles by this author Shaima Abdulrahman [email protected] Bartin Universitesi, Bartın, Turkey, 74100 View all articles by this author Behcet inal [email protected] Bartin Universitesi, Bartın, Turkey, 74100 View all articles by this author Mehmet Balcı [email protected] Bartin Universitesi, Bartın, Turkey, 74100 View all articles by this author Mehmet Cengiz KARAİSMAİLOĞLU [email protected] View all articles by this author Metrics & Citations Metrics Article Usage 9 views 4 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Mehmet Karaismailoğlu, Vahap Eldem, Shaima Abdulrahman, et al. Integrative Taxonomic Delimitation of the Endemic Thlaspi syriacum and Thlaspi microstylum Using Molecular, Micromorphological, and Anatomical Evidences. Authorea . 12 May 2026. DOI: https://doi.org/10.22541/authorea.15003116/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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