S‑adenosyl methionine regulates calcium channels and inhibits uterine smooth muscle contraction in rats with infectious premature delivery through the transient receptor protein 3/protein kinase Cβ/C‑kinase‑activated protein phosphatase‑1 inhibitor of 17�kDa signaling pathway

Jing Ge, Tao Han, Xiaoqiu Li, Lili Shan, Jinhuan Zhang, Yan Hong, Yanqiu Xia, Jun Wang, Mingxiao Hou
In: Experimental and Therapeutic Medicine · 2018 · vol. 16(1) , pp. 103–112 · doi:10.3892/etm.2018.6164 · PMID:29896230 · W2802376026
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Abstract

The aim of the present study was to investigate the effects of S‑adenosyl methionine (SAMe) on infectious premature inflammatory factors and uterine contraction, and to further explore its mechanism of action via the transient receptor protein 3 (TRPC3)/protein kinase Cβ (PKCβ)/C‑kinase‑activated protein phosphatase‑1 inhibitor of 17 kDa (CPI‑17) signaling pathway, following intervention by a TRPC3 inhibitor. A rat model of premature delivery induced by lipopolysaccharide (LPS) was established. Following treatment with SAMe and inhibiting TRPC3 expression, rat serum and uterus were isolated. Hematoxylin and eosin staining was used to observe the histopathological changes in the uterus. Uterine muscle strips in vitro were selected to measure the changes in muscle tension. ELISA was utilized to measure the changes in serum inflammatory factor and oxidative stress indexes. Immunohistochemistry, western blot assay and reverse transcription‑quantitative polymerase chain reaction were applied to detect calcium channel protein expression in the uterus. Western blot analysis was employed to measure the expression of TRPC3/PKCβ/CPI‑17 signaling pathway‑related proteins. TRPC3 was highly expressed in the uterus of rat models of premature delivery induced by LPS. Following treatment with SAMe, inflammatory cell infiltration markedly reduced in the uterus and the tension of in vitro uterine muscle strips significantly decreased. SAMe treatment suppressed inflammatory reaction and oxidative stress, and diminished L‑type and T‑type calcium channel protein expression. TRPC3/PKCβ/CPI‑17 signaling pathway‑related protein expression was also reduced. When TRPC3 expression was suppressed, the effects of SAMe against inflammation and oxidative stress were diminished. TRPC3/PKCβ/CPI‑17 signaling pathway‑related protein expression significantly increased. SAMe was able to reduce inflammatory reaction and oxidative stress in the uterus of rat model of infectious premature delivery induced by LPS, prolong delivery time, reduce the mortality rate of offspring rats, and serve a therapeutic role. This effect is likely achieved via the regulation of uterine contractions and childbirth through the TRPC3/PKCβ/CPI‑17 signaling pathway.

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