Frequency Comb Optoacoustic Tomography

Optoacoustics (OA) is overwhelmingly implemented in the Time Domain (TD) to achieve a high Signal-to-Noise-Ratio (SNR). Implementations in the Frequency Domain (FD) have been proposed, but have not offered competitive advantages over TD methods to reach high dissemination. It is therefore commonly believed that the TD represents the optimal way of performing optoacoustics. Here, we introduce a novel optoacoustic concept based on frequency comb and theoretically demonstrate its superiority to the TD. Then, using recent advances in laser diode illumination, we launch Frequency Comb Optoacoustic Tomography (FCOT), at multiple wavelengths, and experimentally demonstrate its advantages over TD methods in phantoms and in-vivo . We demonstrate that FCOT optimizes the SNR of spectral measurements over TD methods by benefiting from signal acquisition in the TD and processing in the FD, and that it reaches the fastest multi-spectral operation ever demonstrated in optoacoustics while reducing performance compromises present in TD systems.