OBESITY, UREA, URIC ACID: DONKEYING AROUND WITH SUBCLINICAL METABOLIC IMBALANCES (PILOT STUDY)

OBESITY, UREA, URIC ACID: DONKEYING AROUND WITH SUBCLINICAL METABOLIC IMBALANCES (PILOT STUDY) | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 10 February 2025 V1 Latest version Share on OBESITY, UREA, URIC ACID: DONKEYING AROUND WITH SUBCLINICAL METABOLIC IMBALANCES (PILOT STUDY) Authors : Ana Mandić 0009-0002-6268-4412 [email protected] , Bojana Matorkić , Kristina Spariosu , Milena Radaković , Aleksandra Mitrovic 0009-0009-9746-4285 , Katarina Nenadović , and Milica Kovačević Filipović Authors Info & Affiliations https://doi.org/10.22541/au.173920217.77636905/v1 261 views 72 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Background – Obesity is a key precipitating factor in laminitis development and frequent problem in many donkey farms across Europe. Stoic nature of donkeys makes laminitic changes often going unnoticed or mistakenly attributed to hoof neglect. Objectives – To investigate link between obesity, metabolic profile, inflammation, and laminitis, focusing on physical examination and serum biomarkers. Study design – Cross-sectional observational study. Methods – Ten randomly chosen lactating jennies over four years of age, from one farm, being fed with hay and bran, were enrolled in the study. Body condition score (BCS) was assessed using Pearson’s system. Grading scales for fat accumulations and laminitic hoof deformities were developed. Welfare was assessed using AWIN protocol. Blood samples for biochemistry analysis were collected after fasting. Data were presented as median [minimum, maximum] and analysed with MedCalc® software, with p <0.05 considered significant. Results – Laminitic hoof deformities (0.5 [0, 1]), were in strong positive correlation with BCS (7 [4, 8], p =0.008) and fat accumulations (1.5 [0, 2.5], p =0.017). The category of jennies with BCS≥7 had higher insulin than group with BCS<7 ( p =0.044). Insulin (34.86 pmol/L [8.33, 75]) showed positive correlation with glucose (4.12 mmol/L [3.8, 5.4], p <0.001), cholesterol (1.92 mmol/L [1.53, 2.38], p =0.008), and AST (461.6 U/L [379.1, 1037.6], p =0.023). Uric acid (0.20 mmol/L [0.09, 0.62]) showed positive correlation with BCS ( p =0.033) and urea (6.46 mmol/L [3.84, 8.68], p =0.048). Urea levels exceeded reference range in eight, and globulins in all the jennies. Main limitations – Small sample size, unknown gestational state, unawareness of individual food intake. Conclusions – In lactating, normoinsulinaemic and normoglycaemic jennies, over-conditioning is linked to subclinical laminitis. However, clear metabolic link is missing. Increased globulins suggest subclinical chronic inflammation, while the increase in urea and variable levels of UA suggest the need for thorough assessment of proper feeding management. Supplementary Material File (am_manuscript_2025_eve.docx) Download 65.63 KB File (figures_eve.docx) Download 2.56 MB File (tables_eve.docx) Download 20.54 KB Information & Authors Information Version history V1 Version 1 10 February 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords bran globulins insulin jennies laminitis Authors Affiliations Ana Mandić 0009-0002-6268-4412 [email protected] Univerzitet u Beogradu Fakultet veterinarske medicine View all articles by this author Bojana Matorkić Univerzitet u Beogradu Fakultet veterinarske medicine View all articles by this author Kristina Spariosu Univerzitet u Beogradu Fakultet veterinarske medicine View all articles by this author Milena Radaković Univerzitet u Beogradu Fakultet veterinarske medicine View all articles by this author Aleksandra Mitrovic 0009-0009-9746-4285 Univerzitet u Beogradu Fakultet veterinarske medicine View all articles by this author Katarina Nenadović Univerzitet u Beogradu Fakultet veterinarske medicine View all articles by this author Milica Kovačević Filipović Univerzitet u Beogradu Fakultet veterinarske medicine View all articles by this author Metrics & Citations Metrics Article Usage 261 views 72 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Ana Mandić, Bojana Matorkić, Kristina Spariosu, et al. OBESITY, UREA, URIC ACID: DONKEYING AROUND WITH SUBCLINICAL METABOLIC IMBALANCES (PILOT STUDY). Authorea . 10 February 2025. DOI: https://doi.org/10.22541/au.173920217.77636905/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); Cited by Snežana Bulajić, Milica Kovačević-Filipović, Jasna Lončina, Miroslav Kjosevski, Ena Dobric, Katarina Nenadović, A Survey Report on Management Practices on Dairy Donkey Farms in Serbia, Macedonian Veterinary Review, 49 , 1, (103-115), (2026). https://doi.org/10.2478/macvetrev-2026-0014 Crossref Loading... View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. 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