Effects of Crop Rotation with Oat on Soil Microbial Communities, Chemical Properties, and Oat Yield in Southwest China

Effects of Crop Rotation with Oat on Soil Microbial Communities, Chemical Properties, and Oat Yield in Southwest China | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 31 August 2025 V1 Latest version Share on Effects of Crop Rotation with Oat on Soil Microbial Communities, Chemical Properties, and Oat Yield in Southwest China Authors : Xiuyuan Xu , Shuhua Xu , Bo Yuan , Meng Zhu , Jiaqiang Wei , Min Yang , Meji Cuo , … Show All … , Yuehan Shen , Xingfa Gao , Zhenglin Qiao , Xiong Rao , Li-jun Xu [email protected] , and Yingying Nie Show Fewer Authors Info & Affiliations https://doi.org/10.22541/au.175665614.46459855/v1 Published Land Degradation & Development Version of record Peer review timeline 193 views 124 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Oats had become an important crop in southwest China due to their high nutritional value and excellent adaptability to the challenging climate. Continuous oat cropping can lead to reduced oat yield and soil degradation, meanwhile, crop rotation have been proven to be an effective way to alleviate the obstacles of continuous cropping. However, the effects of different crop-oat rotation patterns on oat yield, soil chemical properties and microbial communities in southwest China remain unclear. To address this, we used high-throughput sequencing and quantitative PCR of the 16S and ITS rRNA genes to investigate microbial communities in a multi-pattern crop-oat rotation experiment that from June 2020 to June 2023. The experiment included five treatments, each with five replications: OO (oat-oat), MO (maize-oat), PO (potato-oat), BO (buckwheat-oat), and CO (cabbage-oat). Results showed that crop-oat rotation significantly affected oat yield, soil chemical properties, and microbial communities. The highest oat yield was obtained from the MO and BO treatments. Compared to the OO treatment, the BO treatment had the greatest impact on soil properties and bacterial α-diversity, while fungal α-diversity was highest in the MO and CO treatments. Crop rotation also shifted the soil microbial community structure, the dominant bacterial phyla were Actinobacteriota , Proteobacteria , Chloroflexi , and Acidobacteria , while the dominant fungal phyla were Ascomycota , Basidiomycota , and Mortierellomycota . Principal coordinate analysis (PCoA) showed significant differences in both bacterial and fungal communities between the five treatments. Redundancy analysis (RDA) indicated that soil total nitrogen (TN) and pH were the most important factors shaping the bacterial and fungal communities in oat soil, respectively. The Structural Equation Modeling analysis (SEM) revealed that crop-oat rotation affected the fungal community through its impact on soil organic carbon (SOC), thereby influenced the oat yield. Our findings suggest that buckwheat-oat rotation may be the most suitable crop rotation pattern for enhancing soil quality and oat yield in southwest China, maize-oat is not recommended due to its damage to soil nutrients and microbial communities. Supplementary Material File (manuscript.docx) Download 1.08 MB Information & Authors Information Version history V1 Version 1 31 August 2025 Peer review timeline Published Land Degradation & Development Version of Record 16 Mar 2026 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords crop-oat rotation high-throughput sequencing oat yield soil chemical property soil microorganism Authors Affiliations Xiuyuan Xu Chinese Academy of Agricultural Sciences Institute of Agricultural Resources and Regional Planning View all articles by this author Shuhua Xu Yunnan Agricultural Reclamation Group Co Ltd View all articles by this author Bo Yuan Chinese Academy of Agricultural Sciences Institute of Agricultural Resources and Regional Planning View all articles by this author Meng Zhu Yunnan Agricultural Reclamation Group Co Ltd View all articles by this author Jiaqiang Wei Northeastern University View all articles by this author Min Yang Guizhou Academy of Agricultural Sciences View all articles by this author Meji Cuo Chinese Academy of Agricultural Sciences Institute of Agricultural Resources and Regional Planning View all articles by this author Yuehan Shen Chinese Academy of Agricultural Sciences Institute of Agricultural Resources and Regional Planning View all articles by this author Xingfa Gao Yunnan Agricultural Reclamation Group Co Ltd View all articles by this author Zhenglin Qiao Qujing Medical College View all articles by this author Xiong Rao Qujing Medical College View all articles by this author Li-jun Xu [email protected] Chinese Academy of Agricultural Sciences Institute of Agricultural Resources and Regional Planning View all articles by this author Yingying Nie Chinese Academy of Agricultural Sciences Institute of Agricultural Resources and Regional Planning View all articles by this author Metrics & Citations Metrics Article Usage 193 views 124 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Xiuyuan Xu, Shuhua Xu, Bo Yuan, et al. Effects of Crop Rotation with Oat on Soil Microbial Communities, Chemical Properties, and Oat Yield in Southwest China. Authorea . 31 August 2025. DOI: https://doi.org/10.22541/au.175665614.46459855/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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