Mechanistic Investigation of Cryptotanshinone and Curcumin Combination in Attenuating BVDV-1-Induced Injury in Bovine Ovarian Granulosa Cells.

Chen X, Zhang K, Feng H, Wang L, Zhang J, Lu X, Xu G, Liu S, Feng X, Wang J, Li J
Veterinary sciences · 2026 · doi:10.3390/vetsci13101002 · PMID:42944098 · PMC13709452
Lab / animal OA: gold CC-BY-4.0
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Abstract

Bovine viral diarrhea virus (BVDV) is a widespread pathogen causing significant economic losses in cattle. There is an urgent need for drugs to treat BVDV-induced reproductive dysfunction. This study investigated the mechanisms and targets of the cryptotanshinone-curcumin (CRY-CUR) combination against BVDV-1. Overlapping targets of CRY, CUR, and BVDV were screened using TCMSP, ChEMBL, and GeneCards databases. A Venn diagram was generated, followed by GO and KEGG enrichment analyses via DAVID. A PPI network was constructed using Cytoscape, and molecular docking was performed with AutoDock. Anti-BVDV-1 activity was verified in bovine ovarian granulosa cells (BOGCs) using RT-qPCR and immunofluorescence. A total of 108 overlapping targets were identified, with four core targets (STAT3, TNF, BCL-2, and EGFR) selected. Molecular docking showed strong binding affinities. Enrichment analysis indicated involvement in calcium ion binding, apoptosis, and TNF/NF-κB pathways. In vitro, the CRY-CUR combination suppressed BVDV-1 replication and reversed abnormal mRNA expression of the core targets. The CRY-CUR combination may inhibit BVDV-1 replication, potentially through modulation of BVDV-1-related targets such as STAT3, TNF, BCL-2, and EGFR and their associated signaling pathways.

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chemicals 38
cryptotanshinone curcumin calcium cryptotanshinone curcumin cryptotanshinone curcumin calcium cryptotanshinone cryptotanshinone cryptotanshinone curcumin cryptotanshinone diterpenoid curcumin curcumin curcumin penicillin streptomycin penicillin streptomycin formaldehyde triton penicillin streptomycin formaldehyde calcium calcium hydrogen calcium calcium calcium calcium calcium calcium calcium cryptotanshinone curcumin
organisms 106
pestivirus type 1 bovine viral diarrhea virus strain cp7 pestivirus type 1 oxen pestivirus type 1 pestivirus type 1 pestivirus type 1 pestivirus type 1 pestivirus type 1 pestivirus type 1 pestivirus type 1 bovine viral diarrhea virus strain cp7 oxen pestivirus type 1 bovine viral diarrhea virus strain cp7 pestivirus type 1 oxen multicellular animals wild sheep naine d'afrique de l'ouest deer wild boar pestivirus type 1 oxen pestivirus type 1 oxen pestivirus type 1 pestivirus type 1 pestivirus type 1 pestivirus type 1 oxen multicellular animals oxen pestivirus type 1 salvia miltiorrhiza rattus sp. severe acute respiratory syndrome coronavirus 2 severe acute respiratory syndrome coronavirus 2 prrsv sd 01-08 prrsv pestivirus type 1 zingiberaceae enterovirus l hepatitis b virus subtype adw human immunodeficiency virus type 1 04cd.fr.kzs prrsv sd 01-08 swine fever virus pestivirus type 1 pestivirus type 1 oxen pestivirus type 1 pestivirus type 1 pestivirus type 1 pestivirus type 1 pestivirus type 1 naine d'afrique de l'ouest rabbits pestivirus type 1 pestivirus type 1 suid herpesvirus 1 strain kaplan +46 more

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