Effect of rhPRT on Endometriosis in Rat Model

[Objective] To investigate the effect of recombinant human prolactin (rhPRL) on endometriosis through giving rhPRL to rat endometriosis model.[Method] Fifty rat models with endometriosis were established firstly and were seperated into 6 groups: Study group 1 (10 cases) group 2 (10 cases) and group 3 (10 cases), Danazol control group (10 cases), 0.9% saline control group (6 cases) and empty control group (4 cases). Different concentration [50 μg/(kg·d), 100 μg/(kg·d), 200 μg/(kg·d)] of rhPRL were injected respectively into the abdominal cavity of study groups. Danazol [80 mg/(kg·d)] were given to Danazol controls. Observation index: diameter of nidus were measured by ruler; CD3, CD4, CD8 T lymphocyte were determined by flowcytometry; serum IL-2 and IL-4 were determined by ELISA; the estrus period was indirectly observed by pap smear. [Result] Sixty percent of rat estrus were changed after endometriosis were established, which were controlled by rhPRL. Endometriotic niduses shrinked and adhesions were restricted after the injection of rhPRL. There was no statistic difference between study groups and Danazol control group (P 0.05), but there were statistic differences between study groups and 0.9% saline control group and empty control group (P 0.05). The rate of CD4/CD8 and IL-2 were elevated but IL-4 was lowed in study groups, there were statistic differences between study groups and 0.9% saline control group and empty control group in these indexes (P 0.05).[Conclusion] rhPRL can inhibit the growth and adhesion of endometriosis in rat model through inhibiting the function of gonad and regulating immune function.