Pseudolycorine Chloride Ameliorates Th17-cell-mediated CNS Autoimmunity by Restraining MDSCs Expansion

Pseudolycorine Chloride Ameliorates Th17-cell-mediated CNS Autoimmunity by Restraining MDSCs Expansion | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Pseudolycorine Chloride Ameliorates Th17-cell-mediated CNS Autoimmunity by Restraining MDSCs Expansion Gan Zhang, Xinying Zhu, Fan Yang, Juan Li, Xiao Leng, Limei Li, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-994223/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract In experimental autoimmune encephalomyelitis (EAE), a large number of myeloid-derived suppressor cells (MDSCs) appear, and these cells can accelerate the development of EAE in mice. The aim of this study was to evaluate pseudolycorine chloride’s (PLY) inhibitory effect on the expansion of MDSCs and differentiation of monocyte-like MDSCs (M-MDSCs), while proving that PLY can improve the severity of Th17 cell-mediated EAE by affecting MDSCs. Flow cytometry suggested that PLY significantly inhibited the proliferation and expansion of MDSCs stimulated by IL-6 and GM-CSF, especially the differentiation of M-MDSCs in a dose-dependent manner. Three concentrations of PLY (0.67, 2, and 6 μM) had no cytotoxicity in vitro . Hematoxylin and eosin (H&E) staining, LFB, and immunofluorescence suggested inflammatory infiltration and demyelination. MDSCs and related Th17 cell infiltration in the spinal cord were inhibited by PLY (40 mg/kg). In conclusion, PLY inhibited the differentiation and expansion of MDSCs, especially M-MDSCs, and intervened in the progression of EAE in mice. Clinical Pharmacology MDSCs M-MDSCs Th17 cells experimental autoimmune encephalomyelitis/multiple sclerosis pseudolycorine chloride Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-994223","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":57578847,"identity":"3e02005e-ad75-4095-9198-d65e6b826a40","order_by":0,"name":"Gan Zhang","email":"","orcid":"","institution":"Chengdu Medical College","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Gan","middleName":"","lastName":"Zhang","suffix":""},{"id":57578848,"identity":"9e6f0c4c-047d-44ff-9b9d-83016b3282d1","order_by":1,"name":"Xinying Zhu","email":"","orcid":"","institution":"Chengdu Medical 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chloride","lastPublishedDoi":"10.21203/rs.3.rs-994223/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-994223/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"In experimental autoimmune encephalomyelitis (EAE), a large number of myeloid-derived suppressor cells (MDSCs) appear, and these cells can accelerate the development of EAE in mice. The aim of this study was to evaluate pseudolycorine chloride’s (PLY) inhibitory effect on the expansion of MDSCs and differentiation of monocyte-like MDSCs (M-MDSCs), while proving that PLY can improve the severity of Th17 cell-mediated EAE by affecting MDSCs. Flow cytometry suggested that PLY significantly inhibited the proliferation and expansion of MDSCs stimulated by IL-6 and GM-CSF, especially the differentiation of M-MDSCs in a dose-dependent manner. Three concentrations of PLY (0.67, 2, and 6 μM) had no cytotoxicity in vitro . Hematoxylin and eosin (H\u0026amp;E) staining, LFB, and immunofluorescence suggested inflammatory infiltration and demyelination. MDSCs and related Th17 cell infiltration in the spinal cord were inhibited by PLY (40 mg/kg). In conclusion, PLY inhibited the differentiation and expansion of MDSCs, especially M-MDSCs, and intervened in the progression of EAE in mice.","manuscriptTitle":"Pseudolycorine Chloride Ameliorates Th17-cell-mediated CNS Autoimmunity by Restraining MDSCs Expansion","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2021-10-20 20:05:39","doi":"10.21203/rs.3.rs-994223/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"fae4497e-041c-474e-a097-593da6da5a62","owner":[],"postedDate":"October 20th, 2021","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":7994060,"name":"Clinical Pharmacology"}],"tags":[],"updatedAt":"2021-11-11T19:53:28+00:00","versionOfRecord":[],"versionCreatedAt":"2021-10-20 20:05:39","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-994223","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-994223","identity":"rs-994223","version":["v1"]},"buildId":"7rjqhiLT3MXkJMwkYKINL","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}