Comparative Measurement of Short-Term Fluoride Release and Inhibition of Caries Around Restoration by Ion Releasing Restorative Materials: An In Vitro Study.

Comparative Measurement of Short-Term Fluoride Release and Inhibition of Caries Around Restoration by Ion Releasing Restorative Materials: An In Vitro Study. | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Comparative Measurement of Short-Term Fluoride Release and Inhibition of Caries Around Restoration by Ion Releasing Restorative Materials: An In Vitro Study. Eman T. El-Adl, Maha M. Ebaya, El-Sayed E. Habib, Nadia M. Zaghloul This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4125468/v2 This work is licensed under a CC BY 4.0 License Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Abstract Abstract Objectives: To compare short-term fluoride release of three ion releasing restorative materials and assess their inhibitory effect on secondary caries. Materials and Methods: Self-adhesive hybrid composite (group A), ion releasing flowable composite liner (group B), and alkasite restorative material (group C), were examined. Twenty-two discs were fabricated from each material for fluoride release test, conducted on days 1, 7, and 14. For assessing secondary caries inhibition, sixty-six sound molar teeth were used and standardized class V cavities were prepared. Teeth were divided into three groups according to each material, followed by 2000 cycles of thermocycling. Subsequently, teeth were immersed in a solution containing cariogenic bacteria for 30 days. After that, teeth were sectioned bucco-lingually and analyzed using a polarized light microscope to measure inhibition area, outer lesion depth, and extension. Data was statistically analyzed using different tests. Results: Statistically significant differences in fluoride release existed among materials. Self-adhesive hybrid composite exhibited the highest fluoride release, followed by alkasite restorative material and ion releasing flowable composite liner. Lesion extension and depth were statistically significantly greater next to ion releasing flowable composite liner, while next to alkasite restorative material, they exceeded those next to Self-adhesive hybrid composite. The inhibition areas next to self-adhesive hybrid composite were statistically significantly larger than alkasite restorative material and ion releasing flowable composite liner. There was statistically significant positive correlation between fluoride release and lesion depth and extension at day 1, 7 and 14. Also, there was statistically significant negative correlation between fluoride release and inhibition area at day 1, 7 and 14. Conclusion: All three ion releasing restorative materials displayed fluoride release and potential to inhibit secondary caries formation. Self-adhesive hybrid composite demonstrated the highest fluoride-releasing potential and the greatest ability to inhibit secondary caries. Conversely, ion releasing flowable composite liner exhibited the least fluoride release with minimal secondary caries inhibition. Increasing fluoride release correlated with larger inhibition areas and reduced outer lesion depth and extension. Dentistry Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4125468","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":284174999,"identity":"df2256e8-1728-4445-be24-18a08075d515","order_by":0,"name":"Eman T. 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A: Occlusal lesions with positive birefringence (embedding in water) adjacent to Self-adhesive hybrid resin composite. Lesions were distant from the restoration, resulting in an inhibition area. B: Occlusal Lesion adjacent to Alkasite restorative material, with positive birefringence with inhibition area being visible. C: Occlusal lesions with positive birefringence adjacent to Bioactive flowable resin composite liner, with inhibition area that is very minimal. D: Dentin, E: Enamel, L: Lesion, R: Restoration, IA: Inhibition area.\u003c/strong\u003e\u003c/p\u003e","description":"","filename":"fig4.png","url":"https://assets-eu.researchsquare.com/files/rs-4125468/v2/e80eecf3efcf97386b8735fe.png"},{"id":58141897,"identity":"2f3b1b9e-f31f-4419-bc69-2da3f618d408","added_by":"auto","created_at":"2024-06-11 17:55:37","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":17099,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003eComparisons of lesion parameters between the three materials\u003c/strong\u003e\u003c/p\u003e","description":"","filename":"fig5.png","url":"https://assets-eu.researchsquare.com/files/rs-4125468/v2/1bb2d31da42694d322b3b6c9.png"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eComparative Measurement of Short-Term Fluoride Release and Inhibition of Caries Around Restoration by Ion Releasing Restorative Materials: An In Vitro Study.\u003c/strong\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-4125468/v2","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4125468/v2","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eAbstract\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eObjectives:\u003c/strong\u003e To compare short-term fluoride release of three ion releasing restorative materials and assess their inhibitory effect on secondary caries.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMaterials and Methods:\u003c/strong\u003e Self-adhesive hybrid composite (group A), ion releasing flowable composite liner (group B), and alkasite restorative material (group C), were examined. Twenty-two discs were fabricated from each material for fluoride release test, conducted on days 1, 7, and 14. For assessing secondary caries inhibition, sixty-six sound molar teeth were used and standardized class V cavities were prepared. Teeth were divided into three groups according to each material, followed by 2000 cycles of thermocycling. Subsequently, teeth were immersed in a solution containing cariogenic bacteria for 30 days. After that, teeth were sectioned bucco-lingually and analyzed using a polarized light microscope to measure inhibition area, outer lesion depth, and extension. Data was statistically analyzed using different tests.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults:\u003c/strong\u003e Statistically significant differences in fluoride release existed among materials. Self-adhesive hybrid composite exhibited the highest fluoride release, followed by alkasite restorative material and ion releasing flowable composite liner. Lesion extension and depth were statistically significantly greater next to ion releasing flowable composite liner, while next to alkasite restorative material, they exceeded those next to Self-adhesive hybrid composite. The inhibition areas next to self-adhesive hybrid composite were statistically significantly larger than alkasite restorative material and ion releasing flowable composite liner. There was statistically significant positive correlation between fluoride release and lesion depth and extension at day 1, 7 and 14. Also, there was statistically significant negative correlation between fluoride release and inhibition area at day 1, 7 and 14.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion:\u003c/strong\u003e All three ion releasing restorative materials displayed fluoride release and potential to inhibit secondary caries formation. Self-adhesive hybrid composite demonstrated the highest fluoride-releasing potential and the greatest ability to inhibit secondary caries. Conversely, ion releasing flowable composite liner exhibited the least fluoride release with minimal secondary caries inhibition. Increasing fluoride release correlated with larger inhibition areas and reduced outer lesion depth and extension.\u003c/p\u003e","manuscriptTitle":"Comparative Measurement of Short-Term Fluoride Release and Inhibition of Caries Around Restoration by Ion Releasing Restorative Materials: An In Vitro Study.","msid":"","msnumber":"","nonDraftVersions":[{"code":2,"date":"2024-06-11 17:55:32","doi":"10.21203/rs.3.rs-4125468/v2","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}},{"code":1,"date":"2024-03-26 15:45:46","doi":"10.21203/rs.3.rs-4125468/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"35ba5c24-d88e-404c-9e1f-d84ddec0b959","owner":[],"postedDate":"June 11th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":32977516,"name":"Dentistry"}],"tags":[],"updatedAt":"2024-07-10T04:38:51+00:00","versionOfRecord":[],"versionCreatedAt":"2024-06-11 17:55:32","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v2","identity":"rs-4125468","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4125468","identity":"rs-4125468","version":["v2"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}