Entomoscope 2.0 and ENIMAS 2.0: An Open-Source, AI-Integrated Platform for Rapid and Affordable Insect Digitization

Abstract The rapid decline of global biodiversity necessitates scalable and accessible tools for monitoring insect populations, yet the high cost and slow pace of specimen digitization remain significant bottlenecks. To address this challenge, we present the Entomoscope 2.0, an open-source platform that integrates a low-cost photomicroscope with an AI-integrated software suite, ENIMAS 2.0. The system combines optimized hardware with an end-to-end software module for digital specimen curation. This module comprises automated specimen cropping, background standardization, morphometric analysis using an Oriented Bounding Box (OBB) with a human-in-the-loop (HITL) supervision, and a flexible interface for rapid taxonomic screening using custom AI identification models. With a material cost of only 400 €, the system offers a cost-effective alternative to expensive commercial solutions. We compare results from the Entomoscope 2.0 with a high-end commercial system (Keyence) using 54 insect specimens and demonstrate the efficiency of the proposed AI workflow. Entomoscope 2.0 completed the whole digitization process in an average of 54.6 seconds per specimen, representing a 2.28-fold increase in speed over the Keyence system’s multi-step workflow (124.3 seconds). Crucially, all hardware specifications and construction manuals are freely available to support widespread adoption. By lowering financial barriers and accelerating research workflows, the Entomoscope 2.0 platform offers a practical solution to enable high-throughput digitization for researchers, educators, and citizen scientists. Competing Interest Statement The authors have declared no competing interest.