Preparation of magnetic dendrimer and immobilization of laccase for dye removal

Preparation of magnetic dendrimer and immobilization of laccase for dye removal | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Preparation of magnetic dendrimer and immobilization of laccase for dye removal Tianwen Lu, Yixuan Dai, Yue Shi, Xiaojun Wang, Yinan Hong, Hong Pan, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6529585/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 02 Jul, 2025 Read the published version in BioNanoScience → Version 1 posted 14 You are reading this latest preprint version Abstract Laccase is a kind of green biocatalyst which is widely distributed in nature. However, the application of free laccase was greatly limited due to the poor stability and low utilization. In order to prepare a carrier with abundant immobilized sites, magnetic dendrimer (MPG-x) grafted by PAMAM was constructed using amino-functionalized Fe 3 O 4 as divergent nucleus in this study. Then the immobilized laccase MPG-2@GA-Lac and MPG-2@Cu 2+ -Lac were prepared by glutaraldehyde crosslinking and copper chelating adsorption, respectively. The results showed that the two immobilized laccases showed better comprehensive stability than free laccase. Moreover, the activation of Cu 2+ in the active center of laccase made MPG-2@Cu 2+ -Lac exhibit better stability. MPG-2@GA-Lac and MPG-2@Cu 2+ -Lac retained 74.4% and 108.1% of the initial free laccase activity, respectively. Compared with MPG-2@GA-Lac, MPG-2@Cu 2+ -Lac achieved better methyl orange (MO) removal over a wider pH and temperature range. This study is expected to further expand the application of laccase in the degradation of dye wastewater. magnetic nanoparticles Polyaminoamide (PAMAM) dendrimers immobilized laccase MO removal Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 02 Jul, 2025 Read the published version in BioNanoScience → Version 1 posted Editorial decision: Revision requested 30 May, 2025 Reviews received at journal 29 May, 2025 Reviews received at journal 27 May, 2025 Reviewers agreed at journal 21 May, 2025 Reviews received at journal 19 May, 2025 Reviewers agreed at journal 19 May, 2025 Reviews received at journal 16 May, 2025 Reviewers agreed at journal 14 May, 2025 Reviewers agreed at journal 08 May, 2025 Reviewers agreed at journal 07 May, 2025 Reviewers invited by journal 07 May, 2025 Editor assigned by journal 07 May, 2025 Submission checks completed at journal 07 May, 2025 First submitted to journal 25 Apr, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6529585","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":453841188,"identity":"e2c28712-69da-4886-a048-fda1648a1b74","order_by":0,"name":"Tianwen Lu","email":"","orcid":"","institution":"Xi’an Polytechnic University","correspondingAuthor":false,"prefix":"","firstName":"Tianwen","middleName":"","lastName":"Lu","suffix":""},{"id":453841189,"identity":"8c0cdea5-ad9d-41f5-a47d-3060f32d694b","order_by":1,"name":"Yixuan Dai","email":"","orcid":"","institution":"Xi’an Polytechnic 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However, the application of free laccase was greatly limited due to the poor stability and low utilization. In order to prepare a carrier with abundant immobilized sites, magnetic dendrimer (MPG-x) grafted by PAMAM was constructed using amino-functionalized Fe\u003csub\u003e3\u003c/sub\u003eO\u003csub\u003e4\u003c/sub\u003e as divergent nucleus in this study. Then the immobilized laccase MPG-2@GA-Lac and MPG-2@Cu\u003csup\u003e2+\u003c/sup\u003e -Lac were prepared by glutaraldehyde crosslinking and copper chelating adsorption, respectively. The results showed that the two immobilized laccases showed better comprehensive stability than free laccase. Moreover, the activation of Cu\u003csup\u003e2+\u003c/sup\u003e in the active center of laccase made MPG-2@Cu\u003csup\u003e2+\u003c/sup\u003e -Lac exhibit better stability. MPG-2@GA-Lac and MPG-2@Cu\u003csup\u003e2+\u003c/sup\u003e -Lac retained 74.4% and 108.1% of the initial free laccase activity, respectively. 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