Unveiling The Role of lncRNA NRON and NFATc3 In Type 2 Diabetes Mellitus: A Molecular Insight for Disease Pathogenesis and Biomarker Discovery

Background: Long non-coding RNAs (lncRNAs) are key factors in the progression of many metabolic diseases. This study aimed to investigate the role of long non-coding RNA repressor of the nuclear factor of activated T cells (lncRNA NRON) and nuclear factor of activated T-cells, cytoplasmic 3 (NFATc3) in type-2 diabetes mellitus (T2DM). Methods and Results The expression patterns of NRON and NFATc3 were measured using quantitative real-time PCR and western blotting. Network analysis and gene enrichment analysis were carried out using bioinformatic tools and the Cytoscape platform. The results showed that NRON was upregulated in T2DM patients, while NFATc3 gene and protein accounted for a lower expression ( p  < 0.05). Bioinformatics analysis displayed that these biomarkers play key roles in multiple mechanisms and pathways linked to the development of T2DM. Conclusions lncRNA NRON and NFATc3 can act as potential biomarkers for the diagnosis and prognosis of T2DM.