Epigenetic Regulations, Motif and Pathway Identification of Myelofibrosis Chip Sequences

Next generation sequence technique that is Chromatin immunoprecipitation (ChIP) on the ChIP–Seq of Myelofibrosis. Myelofibrosis is a type of blood cancer which is also considered as a form of chronic leukemia. It is two types primary myelofibrosis and secondary myelofibrosis its leads to complication of an autoimmune disease, additional disease features include hepatosplenomegaly, extramedullary hematopoiesis (EMH) etc. In this work, its chip-Seq is retrieved from SRA database, the PCR duplicates are removed and finally the genome enriched region is identified using the MAC2 call peak tool. This annotated peak table was used to identify the motifs present in the chip-Seq of Myelofibrosis. Peak table was annotated to identify the genes and the corresponding pathways was identified from the KEGG pathway.