In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion
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OA: closed
CC-BY-4.0
Abstract
Abstract Here, we describe viP-CLIP (in vivo PAR-CLIP) that is capable to identify endogenous RBP targets in mammalian tissues, thereby facilitating the functional analysis of RBP-regulatory networks in vivo. Briefly, mice are exposed to 4-Thiouridine that is incorporated into newly transcribed RNAs in the organism, enabling an efficient UV-crosslinking in multiple tissues crucial for a successful experiment. We report a comprehensive protocol of the critical steps to adapt the cell culture PAR-CLIP1 protocol to the viP-CLIP protocol carried out in mice.
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- europepmc
- last seen: 2026-05-19T01:45:01.086888+00:00
- unpaywall
- last seen: 2026-05-26T02:00:01.498150+00:00
License: CC-BY-4.0