Correlation between ovarian follicular development and Hippo pathway in polycystic ovary syndrome

Background: For women of childbearing age, the biggest problem caused by PCOS is infertility, which is mainly caused by anovulation, abnormal follicular development, proliferation of small antral follicles and the appearance of cystic follicles. The mechanism of its occurrence is not clear. The abnormal proliferation and development of follicles in PCOS patients is a complex process, which is affected by many factors. Previous studies have shown that the switch of Hippo pathway can be regulated by cell volume and cell density. Result: We found that compared with the control group, the number of small follicles in DHEA group increased, the expression of ovarian reserve function protein AMH increased, the expression of cell proliferation index protein PCNA decreased, and the expression of related proteins in the upstream and downstream of Hippo pathway increased. After administration of VP, the expression of AMH, an indicator of ovarian reserve function, and the expression of related proteins in the upstream and downstream of Hippo pathway decreased. Conclusion: This study shows that the increase of the number of small follicles in the ovary, the changes of ovarian reserve function and cell proliferation may be closely related to the switch of Hippo pathway, suggesting that the occurrence of PCOS may be closely related to Hippo pathway, and Hippo pathway may be an important pathway affecting the proliferation and development of PCOS follicles.