Xanthohumol promotes cognitive function-associated neuroprotein changes in two mouse models of Alzheimer’s disease

Xanthohumol promotes cognitive function-associated neuroprotein changes in two mouse models of Alzheimer’s disease | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Xanthohumol promotes cognitive function-associated neuroprotein changes in two mouse models of Alzheimer’s disease Wei Liu, Benhong Xu, Xiaohu Ren, Chen Yang, Guowei Li, Zhen Zhang, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8035932/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Alzheimer's disease (AD) is a neurodegenerative disorder that progressively impairs cognitive function for which there is no effective treatment. Natural dietary compounds such as xanthohumol (Xn) potentially offer a new therapeutic approach in AD. We aim to employ quantitative proteomic techniques to investigate the effects of Xn on cognitive impairment in two established murine models of AD. Treatment with 0.5mg/kg Xn significantly improved the ability of stimulating learning and memory and cognitive function of 3×Tg AD mice while 5mg/kg Xn ameliorated cognitive decline in APP/PS1 mice. We identified two common differential proteins and 12 pathways in the hippocampi of 3×Tg AD mice and 70 common differential proteins and 22 pathways in APP/PS1 mice. Moreover, Nine common signaling pathways mainly involved in energy metabolism, neurodegenerative disease, cardiovascular disease and cancer. So multi high-throughput proteomic analyses of hippocampus add to the scientific basis for development of effective drugs for AD, one candidate of which is Xn. Alzheimer’s disease xanthohumol cognitive impairment proteomics tandem mass tags mouse Full Text Additional Declarations No competing interests reported. Supplementary Files XnProteomicsSupplementarymaterials.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8035932","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":544869513,"identity":"b7b3bcee-7560-4880-9eab-8962efb4187e","order_by":0,"name":"Wei 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Natural dietary compounds such as xanthohumol (Xn) potentially offer a new therapeutic approach in AD. We aim to employ quantitative proteomic techniques to investigate the effects of Xn on cognitive impairment in two established murine models of AD. Treatment with 0.5mg/kg Xn significantly improved the ability of stimulating learning and memory and cognitive function of 3\u0026times;Tg AD mice while 5mg/kg Xn ameliorated cognitive decline in APP/PS1 mice. We identified two common differential proteins and 12 pathways in the hippocampi of 3\u0026times;Tg AD mice and 70 common differential proteins and 22 pathways in APP/PS1 mice. Moreover, Nine common signaling pathways mainly involved in energy metabolism, neurodegenerative disease, cardiovascular disease and cancer. 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