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by claude@2026-07, 2026-07-15
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This study investigated matrix metalloproteinases (MMPs) in adenomyosis, finding that MMP-7, MMP-2, and MMP-9 expression correlates with the invasive ability of ectopic endometrial cells.
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by claude@2026-07, 2026-07-15
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The paper investigated the involvement of matrix metalloproteinases (MMP-7, MMP-2, and MMP-9) in the invasive ability of human adenomyosis by comparing cell cultures from adenomyosis, uterine adenocarcinoma, normal uterine stroma, and normal uterine gland. A collagen gel invasion assay showed adenocarcinoma cells were highly invasive, adenomyosis cells moderately invasive, normal stroma had some invasion, and normal gland cells had none; in situ zymography indicated gelatinase activity in adenomyosis and adenocarcinoma but not normal endometrium. Immunohistochemistry and in situ hybridization found tissue- and phase-specific expression patterns, supporting a model in which adenomyosis invasion is associated with glandular MMP-7 and stromal MMP-2/MMP-9, with the caveat that conclusions rely on ex vivo/cell-culture and tissue-expression correlations. This paper is centrally about endometriosis and/or adenomyosis—specifically adenomyosis, focusing on MMP-driven mechanisms of ectopic endometrial cell invasiveness within the uterus.
Abstract
We are presently investigating the involvement of matrix metalloproteinase (MMP-7, MMP-2 and MMP-9) in the invasive ability of human adenomyosis. A collagen gel invasion assay showed that cell cultures from uterine an adenocarcinoma cell line were highly invasive, those from adenomyosis were moderately invasive, those from normal uterine stroma had some invasive ability and those from normal uterine gland had no invasive ability. In situ zymography demonstrated that gelatinase activity was present in adenomyosis and uterine adenocarcinoma, whereas no gelatinase activity was present in normal uterine endometrium. Immunohistochemical study showed that immunoreactive MMP-7 was present on the glandular cells of uterine adenocarcinoma, adenomyosis and normal uterine endometrium, but not on the stromal cells of these tissues. Higher amounts of immunoreactive MMP-7 were present in adenocarcinoma and adenomyosis than in normal uterine endometrium in proliferative phase, whereas immunoreactive MMP-7 was absent in normal uterine endometrium in secretory phase. Immunoreactive MMP-2 and MMP-9 were present in both glandular cells and stromal cells in uterine adenocarcinoma and adenomyosis, whereas those in normal uterine endometrium were weakly expressed in stromal cells whether in the proliferative or secretory phase. An in situ hybridization study showed that MMP-7 mRNA intracellular expression was different according to the tissues used: that in uterine adenocarcinoma cells it was homogenous in cytoplasm; that in adenomyosis it was homogenous in cytoplasm in some cells and was localized at the apical site in other cells, and that in normal uterine endometrium it was localized at the apical site of the cells. The expressions of MMP-2 and MMP-9 mRNAs by in situ hybridization were also characteristic according to the tissues used: MMP-2 and MMP-9 mRNAs in uterine adenocarcima cells were present both in glandular cells and stromal cells; those in adenomyosis were expressed in the stromal cells adjacent to the glandular cells; those in normal endometrium were weakly expressed both in proliferative and secretory phase. The present study is compatible with the idea that the invasive ability of adenomyosis is associated with the glandular expression of MMP-7 and the stromal expression of MMP-2 and MMP-9.
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アイテム
子宮腺筋症における異所性内膜細胞の特性
https://doi.org/10.15114/smj.68.41
https://doi.org/10.15114/smj.68.418aa0a28c-6e10-48d1-ab89-94020894a7ea
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
|
n0036472X68141.pdf (4.0 MB)
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| アイテムタイプ | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2019-07-31 | |||||||||
| タイトル | ||||||||||
| タイトル | 子宮腺筋症における異所性内膜細胞の特性 | |||||||||
| 言語 | ja | |||||||||
| タイトル | ||||||||||
| タイトル | Characteristics of ectopic uterine endometrium in adenomyosis | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Adenomyosis | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Matrix metalloproteinase | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | In situ hybridization | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | In situ zymography | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Invasion assay | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| ID登録 | ||||||||||
| ID登録 | 10.15114/smj.68.41 | |||||||||
| ID登録タイプ | JaLC | |||||||||
| 著者 |
蓼, 英
× 蓼, 英
× 藤井, 美穂
|
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| 著者別名 | ||||||||||
| 識別子Scheme | WEKO | |||||||||
| 識別子 | 25354 | |||||||||
| 姓名 | Liao, Ying | |||||||||
| 著者別名 | ||||||||||
| 識別子Scheme | WEKO | |||||||||
| 識別子 | 25355 | |||||||||
| 姓名 | Fujii, Miho | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | We are presently investigating the involvement of matrix metalloproteinase （MMP-7, MMP-2 and MMP-9） in the invasive ability of human adenomyosis. A collagen gel invasion assay showed that cell cultures from uterine an adenocarcinoma cell line were highly invasive, those from adenomyosis were moderately invasive, those from normal uterine stroma had some invasive ability and those from normal uterine gland had no invasive ability. In situ zymography demonstrated that gelatinase activity was present in adenomyosis and uterine adenocarcinoma, whereas no gelatinase activity was present in normal uterine endometrium. Immunohistochemical study showed that immunoreactive MMP-7 was present on the glandular cells of uterine adenocarcinoma, adenomyosis and normal uterine endometrium, but not on the stromal cells of these tissues. Higher amounts of immunoreactive MMP-7 were present in adenocarcinoma and adenomyosis than in normal uterine endometrium in proliferative phase, whereas immunoreactive MMP-7 was absent in normal uterine endometrium in secretory phase. Immunoreactive MMP-2 and MMP-9 were present in both glandular cells and stromal cells in uterine adenocarcinoma and adenomyosis, whereas those in normal uterine endometrium were weakly expressed in stromal cells whether in the proliferative or secretory phase. An in situ hybridization study showed that MMP-7 mRNA intracellular expression was different according to the tissues used: that in uterine adenocarcinoma cells it was homogenous in cytoplasm; that in adenomyosis it was homogenous in cytoplasm in some cells and was localized at the apical site in other cells, and that in normal uterine endometrium it was localized at the apical site of the cells. The expressions of MMP-2 and MMP-9 mRNAs by in situ hybridization were also characteristic according to the tissues used: MMP-2 and MMP-9 mRNAs in uterine adenocarcima cells were present both in glandular cells and stromal cells; those in adenomyosis were expressed in the stromal cells adjacent to the glandular cells; those in normal endometrium were weakly expressed both in proliferative and secretory phase. The present study is compatible with the idea that the invasive ability of adenomyosis is associated with the glandular expression of MMP-7 and the stromal expression of MMP-2 and MMP-9. | |||||||||
| 書誌情報 |
札幌医学雑誌 = The Sapporo medical journal en : The Sapporo medical journal 巻 68, 号 1, p. 41-50, 発行日 1999-06-01 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0036-472X | |||||||||
| 著者版フラグ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 出版者 | ||||||||||
| 出版者 | 札幌医科大学医学部 |
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