Visual LAMP Method for Detection of Vibrio vulnificus in Aquatic Products and Environmental Water

Background: A visualized, rapid, simple method was developed based on loop-mediated isothermal amplification (LAMP) assay to detect Vibrio vulnificus in aquatic products and aquaculture waters. Results: Genomic DNA was extracted from Vibrio vulnificus using the boiling and column extraction methods and optimized primers were used to detect the gyrB gene using a visual LAMP method. The sensitivity of the assay was 10 fg/μL, and the results were stable and reliable. Of 655 aquatic product samples and 558 aquaculture waters samples, the positive rates of Vibrio vulnificus detection were 9.01% and 8.60%, respectively, which is markedly higher than that of the traditional culture identification methods. Conclusion: The relatively simple technical requirements, low equipment costs, and rapid detection time make the visual LAMP method for detection of Vibrio vulnificus a convenient choice for field diagnosis in the aquaculture industry.