A simple liquid culture method provides a novel tool for biotechnology

Abstract We propose a simple tool for liquid static culture using a copolymer film with high gas permeability. The film bags were successfully used to culture microorganisms Escherichia coli, Komagataella phaffii (methylotrophic) and Bacillus sp. (biofilm-forming), with cells cultured under physical stress-free conditions with sufficient oxygen supply. Direct oxygen supply from the film surface to the attached cells may be important for successful culturing.