Study on the effect of hydrogen sulfide on oxidative stress in renal tubular epithelial cells injured by hypoxia-reoxygenation

Abstract Background: The present study was designed to determine whether exogenous hydrogen sulfide (H 2 S) inhibits oxidative stress in renal tubular epithelial cells under hypoxia-reoxygenation injury. Material and Methods: NRK-52E cells were divided into 4 group, which were Sham group, HR group, NaHS group and DPI group. Using western blotting to detect the protein expression of NOX2 and NOX4 in the process of hypoxia-reoxygenation. Using real-time quantitative PCR to detect the NOX2 and NOX4 mRNA expression in hypoxia-reoxygenation. The fluorescent probe DCFH-DA was used to detect the content of ROS (reactive oxygen species) in cells. The activity of NADPH oxidase was detected by enzyme-linked immunosorbent assay (ELISA). Results: The protein and mRNA expression of NOX2 and NOX4 were significantly increased in the process of hypoxia-reoxygenation, but decreased with NaHS intervention. ROS content and NADPH oxidase activity were all increased in hypoxia-reoxygenation, but decreased after NaHS intervention. Conclusions: H 2 S can reduce ROS production by inhibiting NOX4 and NOX2 during oxidative stress, and also directly inhibits NADPH oxidase activity.