Molecular Identification by mtDNA and Selection of Morphometric Variables of Haplotypes of Meloidogyne arenaria in Colombia: A statistical computational approach

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Abstract

Root-knot nematodes are ubiquitous parasites with an amazing ability to interact with a wide variety of plant species. Meloidogyne species are recognized worldwide as a major cause of yield limitation in crops. In two Colombian crops, lulo and plantain, root-knot nematodes were identified using morphometric and molecular methods, with the latter using amplification and sequencing of mitochondrial DNA NADH dehydrogenase subunit 5 genes. For M. arenaria, two haplotypes were identified, one associated with plantain and the other with lulo. To select a smaller set of variables for discriminating crops, an algorithm was developed using Hotelling's T2 test in different scenarios generated by the matrix of variances and covariances of the statistic, as well as by the method of estimation. The most important result was associated with the ability to discriminate haplotypes of nematodes species using relationships between body length, tail length and body diameter at the anal opening, which can simplify the complexity of morphometric measurements to characterize banana and lulo crop nematodes.
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Molecular Identification by mtDNA and Selection of Morphometric Variables of Haplotypes of Meloidogyne arenaria in Colombia: A statistical computational approach | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 14 April 2025 V1 Latest version Share on Molecular Identification by mtDNA and Selection of Morphometric Variables of Haplotypes of Meloidogyne arenaria in Colombia: A statistical computational approach Authors : Miguel Acosta , Eyder Gómez , aquiles darghan 0000-0001-5790-1684 [email protected] , and Carlos Rivera Authors Info & Affiliations https://doi.org/10.22541/au.174465607.73047919/v1 196 views 124 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Root-knot nematodes are ubiquitous parasites with an amazing ability to interact with a wide variety of plant species. Meloidogyne species are recognized worldwide as a major cause of yield limitation in crops. In two Colombian crops, lulo and plantain, root-knot nematodes were identified using morphometric and molecular methods, with the latter using amplification and sequencing of mitochondrial DNA NADH dehydrogenase subunit 5 genes. For M. arenaria, two haplotypes were identified, one associated with plantain and the other with lulo. To select a smaller set of variables for discriminating crops, an algorithm was developed using Hotelling's T2 test in different scenarios generated by the matrix of variances and covariances of the statistic, as well as by the method of estimation. The most important result was associated with the ability to discriminate haplotypes of nematodes species using relationships between body length, tail length and body diameter at the anal opening, which can simplify the complexity of morphometric measurements to characterize banana and lulo crop nematodes. Supplementary Material File (nmfig1.docx) Download 58.16 KB File (nmfig2.docx) Download 1.22 MB File (vs_nematodes_28pp_with_data_share (1).docx) Download 29.30 MB Information & Authors Information Version history V1 Version 1 14 April 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords agriculture bioinfomatics/phyloinfomatics biotechnology worms Authors Affiliations Miguel Acosta Universidad Nacional de Colombia - Campus Palmira View all articles by this author Eyder Gómez Universidad Nacional de Colombia - Campus Palmira View all articles by this author aquiles darghan 0000-0001-5790-1684 [email protected] Universidad Nacional de Colombia View all articles by this author Carlos Rivera Universidad Nacional de Colombia View all articles by this author Metrics & Citations Metrics Article Usage 196 views 124 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Miguel Acosta, Eyder Gómez, aquiles darghan, et al. Molecular Identification by mtDNA and Selection of Morphometric Variables of Haplotypes of Meloidogyne arenaria in Colombia: A statistical computational approach. Authorea . 14 April 2025. DOI: https://doi.org/10.22541/au.174465607.73047919/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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