ACP5, TNF, and MMP8 were identified as potential biomarkers of steroid-induced osteonecrosis of the femoral head

ACP5, TNF, and MMP8 were identified as potential biomarkers of steroid-induced osteonecrosis of the femoral head | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article ACP5, TNF, and MMP8 were identified as potential biomarkers of steroid-induced osteonecrosis of the femoral head Jian Zhang, Zehan Liu, Shuai Ren, Zilong Shen, Kecheng Han, Weiguang Xin, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-94892/v2 This work is licensed under a CC BY 4.0 License Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Abstract Steroid-induced osteonecrosis of the femoral head (SONFH) is a progressive bone disorder that is characterized by femoral head collapse and hip joint dysfunction. To elucidate the biomarkers of SONFH, the GSE123568 dataset was downloaded from the Gene Expression Omnibus (GEO) database. A total of 436 differentially expressed SONFH genes were screened in comparison with non-SONFH genes. Six biological processes and four KEGG pathways were enriched in SONFH by GSEA, and 68 candidate genes that were involved in these pathways were selected for subsequent analysis. Moreover, through an ingenuity pathway analysis, we obtained 10 canonical pathways and 20 molecule function modules related to SONFH, and acquired 121 candidate genes. Furthermore, we identified ACP5, TNF, and MMP8 as the genes most related to SONFH according to the VarElect and MalaCards database. Based on these hub genes, the targeted miRNAs and the lncRNAs were predicted. Finally, the ceRNA network was constructed by using ACP5, TNF, MMP8, seven miRNAs, and 956 candidate lncRNAs. In conclusion, the ACP5, TNF, and MMP8 might be potential biomarkers of SONFH. Bioinformatics Steroid-induced osteonecrosis of the femoral head Candidate genes ceRNA network Bioinformatics Full Text Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-94892","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":29415817,"identity":"180f53fa-e66e-447e-8cda-bc3ac985be49","order_by":0,"name":"Jian Zhang","email":"","orcid":"","institution":"Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Jian","middleName":"","lastName":"Zhang","suffix":""},{"id":29667055,"identity":"876d1d04-48e4-48e2-91ba-48ac52d9d158","order_by":1,"name":"Zehan Liu","email":"","orcid":"","institution":"Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Zehan","middleName":"","lastName":"Liu","suffix":""},{"id":29667056,"identity":"0139d9e7-9bf7-4a19-89a3-9217c83a5248","order_by":2,"name":"Shuai Ren","email":"","orcid":"","institution":"Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Shuai","middleName":"","lastName":"Ren","suffix":""},{"id":29667057,"identity":"b39762cf-429e-4d91-aa90-61a5fc52c786","order_by":3,"name":"Zilong Shen","email":"","orcid":"","institution":"Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Zilong","middleName":"","lastName":"Shen","suffix":""},{"id":29667058,"identity":"760532fd-865f-4e71-a24e-98c60c9138be","order_by":4,"name":"Kecheng Han","email":"","orcid":"","institution":"Department of Orthopedics, The fifth Hospital of Harbin","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Kecheng","middleName":"","lastName":"Han","suffix":""},{"id":29667059,"identity":"67383c7d-48ce-4038-8c8d-fa2d4dcb7906","order_by":5,"name":"Weiguang Xin","email":"","orcid":"","institution":"Department of Orthopedics,The Second Hospital of Heilongjiang Province","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Weiguang","middleName":"","lastName":"Xin","suffix":""},{"id":29667060,"identity":"06048e32-d7eb-4635-b91a-c807bbb89c34","order_by":6,"name":"Guanyi He","email":"","orcid":"","institution":"Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Guanyi","middleName":"","lastName":"He","suffix":""},{"id":29667061,"identity":"9e9d243b-9e26-4682-98af-9a16e3625a76","order_by":7,"name":"Chi Huang","email":"","orcid":"","institution":"Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Chi","middleName":"","lastName":"Huang","suffix":""},{"id":29667062,"identity":"3989aab2-3c8f-411a-8f53-ea3eef7a6787","order_by":8,"name":"Jianyu Liu","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA0UlEQVRIiWNgGAWjYBACPmYGBoMEMPPwgQMffhChhQ2uhfFY4sGZPcRogbOYzxgf5mDDoxSuhZ3HoOBBzZ3EDcfOfDjMwMMgzy92gJDDeAwMEo49S9xw5uyGwwUWDIYzZycQo4XtcOKGG0AtM3gYEgxuE6XlH1DL/TcPDvOwEaslsQ2o5cAZBmK1sBUYJPYdNp554JgBMJAlCPuFn//wNsMf3w7L9h04/PjDhx828vzSBLSALDIAEo4NEI4EQeUgwPwASNgTpXQUjIJRMApGJgAAz1FKUXxWQbQAAAAASUVORK5CYII=","orcid":"","institution":"Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Jianyu","middleName":"","lastName":"Liu","suffix":""}],"badges":[],"createdAt":"2020-10-19 15:49:16","currentVersionCode":2,"declarations":"","doi":"10.21203/rs.3.rs-94892/v2","doiUrl":"https://doi.org/10.21203/rs.3.rs-94892/v2","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":13636715,"identity":"3bb0959a-3894-49cf-99d8-542210bdfaef","added_by":"auto","created_at":"2021-09-17 08:43:02","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1104701,"visible":true,"origin":"","legend":"","description":"","filename":"ms.pdf","url":"https://assets-eu.researchsquare.com/files/rs-94892/v2_covered.pdf"},{"id":9664442,"identity":"77794637-b444-4798-9979-6cfa9ff2b63c","added_by":"auto","created_at":"2021-05-27 14:24:37","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1101113,"visible":true,"origin":"","legend":"","description":"","filename":"ms.pdf","url":"https://assets-eu.researchsquare.com/files/rs-94892/v2_covered.pdf"}],"financialInterests":"","formattedTitle":"\u003cp\u003eACP5, TNF, and MMP8 were identified as potential biomarkers of steroid-induced osteonecrosis of the femoral head\u003c/p\u003e","fulltext":[{"header":"Full Text","content":"This preprint is available for \u003ca href='/article/rs-94892/latest.pdf' target='_blank'\u003edownload as a PDF\u003c/a\u003e."}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Steroid-induced osteonecrosis of the femoral head, Candidate genes, ceRNA network, Bioinformatics","lastPublishedDoi":"10.21203/rs.3.rs-94892/v2","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-94892/v2","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eSteroid-induced osteonecrosis of the femoral head (SONFH) is a progressive bone disorder that is characterized by femoral head collapse and hip joint dysfunction. To elucidate the biomarkers of SONFH, the GSE123568 dataset was downloaded from the Gene Expression Omnibus (GEO) database. A total of 436 differentially expressed SONFH genes were screened in comparison with non-SONFH genes. Six biological processes and four KEGG pathways were enriched in SONFH by GSEA, and 68 candidate genes that were involved in these pathways were selected for subsequent analysis. Moreover, through an ingenuity pathway analysis, we obtained 10 canonical pathways and 20 molecule function modules related to SONFH, and acquired 121 candidate genes. Furthermore, we identified ACP5, TNF, and MMP8 as the genes most related to SONFH according to the VarElect and MalaCards database. Based on these hub genes, the targeted miRNAs and the lncRNAs were predicted. Finally, the ceRNA network was constructed by using ACP5, TNF, MMP8, seven miRNAs, and 956 candidate lncRNAs. In conclusion, the ACP5, TNF, and MMP8 might be potential biomarkers of SONFH.\u003c/p\u003e","manuscriptTitle":"ACP5, TNF, and MMP8 were identified as potential biomarkers of steroid-induced osteonecrosis of the femoral head","msid":"","msnumber":"","nonDraftVersions":[{"code":2,"date":"2021-05-27 14:24:29","doi":"10.21203/rs.3.rs-94892/v2","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}},{"code":1,"date":"2020-10-22 14:21:55","doi":"10.21203/rs.3.rs-94892/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"4d4ec675-0ffe-43ee-a6bb-aedaa0b1e5ca","owner":[],"postedDate":"May 27th, 2021","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":4623129,"name":"Bioinformatics"}],"tags":[],"updatedAt":"2020-11-19T18:30:54+00:00","versionOfRecord":[],"versionCreatedAt":"2021-05-27 14:24:29","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v2","identity":"rs-94892","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-94892","identity":"rs-94892","version":["v2"]},"buildId":"cBFmMYwuxLRRLfASyISRj","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}