Effect of 17#beta#--estradiol on secretion of the chemokines IL--8,MIP--1#alpha#,RANTES and I--309 by endometrial stromal cells in vitro

To elucidate the role of estradiol in the pathogenesis and development of endometriosis by exploring the effects of 17#beta#-estradiol on IL-8,MIP-1#alpha#, RANTES and I-309 secretion by endometrial stromal cells. IL-8,MIP-1#alpha#,RANTES and I-309 protein in culture supernatant of endometrial stromal cells after treated by 17#beta#-estradiol with different concentrations for 48 h or for 12-48 h with 10~(-9) mol/L were quantified using ELISA kits of R&D systems. The endometrial stromal cells in vitro secretion in a concentration-dependent manner (P<0.05), but did not affect RANTES secretion. Human endometrial stromal cells did not secrete the chemokine I-309 protein whether treated with estradiol or not. The estradiol may recruit endometrial macrophage and leukocytes migration by regulating IL-8 and MIP-1#alpha# secretion of endometrial stromal cells,and alone is not account for the increased expression of IL-8,RANTES and MIP-1#alpha# in the ectopic tissue of endometriosis.