Standardization and Comparative Evaluation of Laser Fluorimetry for Uranium Detection in Water Sources

Standardization and Comparative Evaluation of Laser Fluorimetry for Uranium Detection in Water Sources | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Standardization and Comparative Evaluation of Laser Fluorimetry for Uranium Detection in Water Sources Jaswinder Kaur, Deep Shikha, Chandan Kapil, Vimal Mehta This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7485922/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract This study standardizes LED fluorimetry for uranium detection in drinking water and compares its performance with inductively coupled plasma mass spectrometry (ICP-MS) and X-ray fluorescence (XRF). Uranium concentrations were measured in 15 samples, comprising 12 water samples from Rupnagar district, Punjab (India), and three standard solutions prepared from certified reference materials. Concentrations ranged from 1.4 to 1000 ppb. LED fluorimetry results showed excellent agreement with ICP-MS (R² = 0.99), confirming its analytical reliability. The technique exhibited a linear response (R² = 0.9986) and maintained an accuracy of ± 10% across a wide dynamic range (0.5–1000 ppb). In contrast, XRF failed to detect uranium below 300 ppb, reflecting its operational limitations to the ppm range and lack of sensitivity for low-level quantification. Owing to its low cost, operational simplicity, and portability, LED fluorimetry offers a practical alternative to advanced laboratory-based methods. It is especially suited for routine uranium monitoring in groundwater, field surveys, and resource-limited settings where access to sophisticated instrumentation is restricted. These findings highlight LED fluorimetry as a reliable and accessible technique for environmental radiation monitoring and public health protection. uranium correlation comparative water techniques Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7485922","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":510391828,"identity":"a2c1bde5-264c-4af8-bc5a-1a406e4dc9a4","order_by":0,"name":"Jaswinder Kaur","email":"","orcid":"","institution":"Punjabi University","correspondingAuthor":false,"prefix":"","firstName":"Jaswinder","middleName":"","lastName":"Kaur","suffix":""},{"id":510391830,"identity":"4787a555-2636-4d7e-b37d-7f57c4b6e3a4","order_by":1,"name":"Deep Shikha","email":"","orcid":"","institution":"Sri Guru Teg Bahadur Khalsa College","correspondingAuthor":false,"prefix":"","firstName":"Deep","middleName":"","lastName":"Shikha","suffix":""},{"id":510391831,"identity":"ce8b1360-7b69-453f-8d98-755cf4c070fe","order_by":2,"name":"Chandan Kapil","email":"","orcid":"","institution":"Punjabi University","correspondingAuthor":false,"prefix":"","firstName":"Chandan","middleName":"","lastName":"Kapil","suffix":""},{"id":510391833,"identity":"42fef7f5-da6d-4972-aef5-7a37cfc52e03","order_by":3,"name":"Vimal Mehta","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA90lEQVRIiWNgGAWjYDACZiB++M+GgUECyPgAxGzsxGhJYEsDamFmYJwB0sJMjE0JbIfBWph5YIbgAwbHuRM/JPCkRfNL9x/8bPNrmzwf0LYPH3PwaDnMu1kiQcImd+acw8zSuX23DduAtknO3IZbi2Qz7waJBIO03A03khmkc3tuMwK1sDHz4tey+UdCwuHc/TeSmX9b9ty2J6iFHygrkXDgcO4GiWQ2aYYftxOJ0mKR2JCWO+NGspllb8Pt5DZmxma8fmHjP7v5xscGm9z+GYmPb/z4c9t2fnvzwQ8f8WhBBYxtYLKBWPUg8IcUxaNgFIyCUTBSAAAji0+OWxvGFgAAAABJRU5ErkJggg==","orcid":"","institution":"Sri Guru Teg Bahadur Khalsa College","correspondingAuthor":true,"prefix":"","firstName":"Vimal","middleName":"","lastName":"Mehta","suffix":""}],"badges":[],"createdAt":"2025-08-29 07:23:21","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7485922/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7485922/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":97139831,"identity":"d80b92ae-bed3-4774-8007-e72b89bb4552","added_by":"auto","created_at":"2025-12-01 10:02:41","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":684152,"visible":true,"origin":"","legend":"","description":"","filename":"EMASStandardizationandComparativeEvaluation.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7485922/v1_covered_184a3105-6399-4f06-90cf-36f64d843351.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Standardization and Comparative Evaluation of Laser Fluorimetry for Uranium Detection in Water Sources","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"uranium, correlation, comparative, water, techniques","lastPublishedDoi":"10.21203/rs.3.rs-7485922/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7485922/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThis study standardizes LED fluorimetry for uranium detection in drinking water and compares its performance with inductively coupled plasma mass spectrometry (ICP-MS) and X-ray fluorescence (XRF). Uranium concentrations were measured in 15 samples, comprising 12 water samples from Rupnagar district, Punjab (India), and three standard solutions prepared from certified reference materials. Concentrations ranged from 1.4 to 1000 ppb. LED fluorimetry results showed excellent agreement with ICP-MS (R\u0026sup2; = 0.99), confirming its analytical reliability. The technique exhibited a linear response (R\u0026sup2; = 0.9986) and maintained an accuracy of \u0026plusmn;\u0026thinsp;10% across a wide dynamic range (0.5\u0026ndash;1000 ppb). In contrast, XRF failed to detect uranium below 300 ppb, reflecting its operational limitations to the ppm range and lack of sensitivity for low-level quantification. Owing to its low cost, operational simplicity, and portability, LED fluorimetry offers a practical alternative to advanced laboratory-based methods. It is especially suited for routine uranium monitoring in groundwater, field surveys, and resource-limited settings where access to sophisticated instrumentation is restricted. 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