Experiments And Simulations of Thermometric Lateral Flow Immunoassay For Point-of-Care Testing

Experiments And Simulations of Thermometric Lateral Flow Immunoassay For Point-of-Care Testing | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Experiments And Simulations of Thermometric Lateral Flow Immunoassay For Point-of-Care Testing Terumitsu Azuma, Yuen Yung Hui, Oliver Y. Chen, Yuh-Lin Wang, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1140710/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 8 You are reading this latest preprint version Abstract Temperature sensing is a promising method of enhancing the detection sensitivity of lateral flow immunoassay for point-of-care testing. A temperature increase of more than 100 °C can be readily achieved by photoexcitation of reporters like gold nanoparticles (GNPs) or colored latex beads (CLBs) on the strips with a laser power below 100 mW. Despite its promise, processes involved in the photothermal detection have not yet been well-characterized. Here, we provide a fundamental understanding of this thermometric assay by combining experiments and simulations using non-fluorescent CLBs as the reporters deposited on nitrocellulose membrane. By measuring the dependence of temperature rises on the number density of membrane-bound CLBs, we determined a 1.5-fold enhancement of the light absorption at 520 nm by the beads (diameter of 0.4 μm). The enhancement, however, was compromised by a 5-fold reduction of the incident laser power due to multiple scattering of the light in this highly porous medium. The limit of detection was measured to be 1 × 10 5 particles/mm 2 . In line with previous studies using GNPs as the reporters, the CLB-based thermometric assay provides a 10× higher sensitivity than color visualization, as demonstrated with the immunoassay for nucleocapsid proteins of the SARS-CoV-2 virus. Materials Engineering Materials Chemistry detection immunoassay sensitivity virus Full Text Additional Declarations No competing interests reported. Supplementary Files SR.SI.pdf Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Major revision 14 Jan, 2022 Reviews received at journal 24 Dec, 2021 Reviewers agreed at journal 16 Dec, 2021 Reviewers invited by journal 16 Dec, 2021 Editor assigned by journal 11 Dec, 2021 Editor invited by journal 09 Dec, 2021 Submission checks completed at journal 09 Dec, 2021 First submitted to journal 04 Dec, 2021 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1140710","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":68935798,"identity":"12e81709-ce4c-4128-9f13-b8eacfedb576","order_by":0,"name":"Terumitsu Azuma","email":"","orcid":"","institution":"Academia Sinica","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Terumitsu","middleName":"","lastName":"Azuma","suffix":""},{"id":68935799,"identity":"f575f395-0b9b-4bc0-9da6-25775ab41409","order_by":1,"name":"Yuen Yung Hui","email":"","orcid":"","institution":"Academia Sinica","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yuen","middleName":"Yung","lastName":"Hui","suffix":""},{"id":68935800,"identity":"e2698237-2ce8-4ff6-aa26-8026b1ae4e21","order_by":2,"name":"Oliver Y. 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